Recombinant Human Protein amnionless (AMN), partial

Code CSB-YP880128HU
MSDS
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Source Yeast
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Code CSB-EP880128HU
MSDS
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Source E.coli
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Code CSB-EP880128HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP880128HU
MSDS
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Source Baculovirus
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Code CSB-MP880128HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AMN
Uniprot No.
Alternative Names
5033428N14Rik; amn; Amnion associated transmembrane protein; Amnionless; Amnionless homolog; AMNLS_HUMAN; AV002116; PRO1028; Protein amnionless; Type I transmembrane protein; UNQ513/PRO1028; Visceral endoderm specific type 1 transmembrane protein
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Membrane-bound component of the endocytic receptor formed by AMN and CUBN. Required for normal CUBN glycosylation and trafficking to the cell surface. The complex formed by AMN and CUBN is required for efficient absorption of vitamin B12. Required for normal CUBN-mediated protein transport in the kidney (Probable).
Gene References into Functions
  1. Study shows that cubilin mutations (novel and some previously reported) and all previously reported amnionless missense mutations resulted in endoplasmic reticulum retention and completely inhibited amnionless-dependent plasma membrane expression of cubilin. PMID: 29402915
  2. heterozygous mutations in AMN in a family from the United Kingdom with clinical features of Imerslund-Grasbeck Syndrome PMID: 26040326
  3. Our genetic screening of 154 families of patients with inherited cobalamin malabsorption revealed population-specific mutations, mutational hotspots, and functionally distinct regions in the three causal genes: CUBN, AMN, and GIF. PMID: 22929189
  4. We present evidence that this founder mutation causes over 50% of the Imerslund-Grasbeck syndrome (IGS) cases among Arabic, Turkish, and Sephardic Jewish families, and the mutation is as old as human civvlization. PMID: 22078000
  5. amnionless is essential for the correct luminal expression of cubilin in humans. PMID: 21750092
  6. homozygous mutations affecting exons 1-4 of human AMN lead to megaloblastic anemia 1 PMID: 12590260
  7. cubilin and amnionless are subunits of a novel cubilin/amnionless (cubam) complex PMID: 14576052
  8. Recurrent spontaneous abortions may be caused by mutations in the Amnionless gene. PMID: 16403802
  9. This review summarizes recent data on the biological function of amnionless and focuses on its implication in embryonic nutrition and central nervous system malformations. PMID: 17979745

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Involvement in disease
Recessive hereditary megaloblastic anemia 1 (RH-MGA1)
Subcellular Location
[Isoform 1]: Apical cell membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Endosome membrane. Membrane, coated pit.; [Soluble protein amnionless]: Secreted.
Tissue Specificity
Detected in proximal tubules in the kidney cortex (at protein level). Long isoforms are highly expressed in small intestine, colon and kidney (renal proximal tubule epithelial cells). Shorter isoforms are detected at lower levels in testis, thymus and per
Database Links

HGNC: 14604

OMIM: 261100

KEGG: hsa:81693

STRING: 9606.ENSP00000299155

UniGene: Hs.534494

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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