ARFGAP3 Antibody

Datasheet

Code	CSB-PA885676ESR1HU
Size	US$166
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Image	Western blot All lanes: ARFGAP3 antibody at 4μg/ml Lane 1: HepG2 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 57, 52 kDa Observed band size: 57 kDa Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA885676ESR1HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA885676ESR1HU at dilution of 1:100 Immunoprecipitating ARFGAP3 in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of (1μg) instead of CSB-PA885676ESR1HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: CSB-PA885676ESR1HU (4μg) + HepG2 whole cell lysate (500μg) Lane 3: HepG2 whole cell lysate (20μg)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ARFGAP3 Polyclonal antibody

Uniprot No.

Q9NP61

Target Names

ARFGAP3

Alternative Names

ADP ribosylation factor GTPase activating protein 1 antibody; ADP ribosylation factor GTPase activating protein 3 antibody; ADP-ribosylation factor GTPase-activating protein 3 antibody; ARF GAP 3 antibody; ARFG3_HUMAN antibody; ArfGAP 3 antibody; ARFGAP1 antibody; ARFGAP3 antibody; FLJ45618 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human ADP-ribosylation factor GTPase-activating protein 3 protein (217-516AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.

Gene References into Functions

We suggest a model in which ArfGAP3 regulates Golgi association of GGA clathrin adaptors. PMID: 24076238
our results suggest that ARFGAP3 is a novel androgen-regulated gene that can promote prostate cancer cell proliferation and migration in collaboration with paxillin. PMID: 21647875
human ARFGAP2 and ARFGAP3 are associated with COP-I-coated vesicles and function in COP I traffic PMID: 17760859
Differential roles of ArfGAP1, ArfGAP2, and ArfGAP3 in COPI trafficking PMID: 19015319
ArfGAP1, ArfGAP2, and ArfGAP3 have overlapping roles in regulating COPI function in Golgi-to-ER retrograde transport. PMID: 19299515

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Subcellular Location

Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment.

Tissue Specificity

Widely expressed. Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate) and testis with a much higher expression in the testis than in the ovary.

Database Links

HGNC: 661

OMIM: 612439

KEGG: hsa:26286

STRING: 9606.ENSP00000263245

UniGene: Hs.685225

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Endocytosis

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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