ARFIP2 Antibody

Code CSB-PA002003LA01HU
Size US$166
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Image
  • Western blot
    All lanes: ARFIP2 antibody at 2µg/ml + Mouse brain tissue
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 38, 30, 34 kDa
    Observed band size: 38 kDa

  • Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA002003LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA002003LA01HU at dilution of 1:100

  • Immunofluorescence staining of Hela cells with CSB-PA002003LA01HU at 1:120,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) ARFIP2 Polyclonal antibody
Uniprot No.
Target Names
ARFIP2
Alternative Names
ADP ribosylation factor interacting protein 2 antibody; ADP-ribosylation factor-interacting protein 2 antibody; Arfaptin 2 antibody; Arfaptin-2 antibody; ARFIP2 antibody; ARFP2_HUMAN antibody; FLJ18046 antibody; FLJ18697 antibody; FLJ99239 antibody; Partner of RAC1 antibody; POR1 antibody; Protein POR1 antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human Arfaptin-2 protein (20-295AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The ARFIP2 Antibody (Product code: CSB-PA002003LA01HU) is Non-conjugated. For ARFIP2 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA002003LB01HU ARFIP2 Antibody, HRP conjugated ELISA
FITC CSB-PA002003LC01HU ARFIP2 Antibody, FITC conjugated
Biotin CSB-PA002003LD01HU ARFIP2 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Plays a role in constitutive metalloproteinase (MMP) secretion from the trans Golgi network. May have important functions during vesicle biogenesis at certain cargo subdomains, which could be predominantly utilized by secreted MMPs, such as MMP7 and MMP2. Also involved in autophagy by regulating the starvation-dependent trafficking of ATG9A vesicles which deliver the phosphatidylinositol 4-kinase beta (PI4KB) to the autophagosome initiation site. In addition, plays a role in NF-kappa-B inhibition by interacting with IKBKB and IKBKG.
Gene References into Functions
  1. The data indicate that arfaptin 2 is the first molecule to regulate NF-kappaB signaling by interacting with the functional IkappaB kinase complex but not by direct inhibiting IKKbeta phosphorylation. PMID: 26296658
  2. both Arf1 activation and high membrane curvature are required for efficient recruitment of Arfaptin2 to membranes PMID: 23638170
  3. The binding of arfaptin1, but not arfaptin2, to PI(4)P is regulated by protein kinase D (PKD) mediated phosphorylation at Ser100 within the AH. PMID: 23695357
  4. The Arl1.Arfaptin-2 BAR structure suggests that one of the two Arl1 molecules competes with Rac1, which binds to the concave face of the Arfaptin-2 BAR homodimer and may hinder its membrane association. PMID: 22679020
  5. arfaptin 2 is involved in regulating huntingtin protein aggregation PMID: 11854752
  6. arfaptin 2 phosphorylation at Ser260 by Akt inhibits PolyQ-huntingtin-induced toxicity by rescuing proteasome impairment PMID: 15809304

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Subcellular Location
Golgi apparatus. Golgi apparatus, trans-Golgi network membrane.
Database Links

HGNC: 17160

OMIM: 601638

KEGG: hsa:23647

STRING: 9606.ENSP00000254584

UniGene: Hs.732203

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