ARHGAP18 Antibody

Datasheet

Code	CSB-PA850791LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, Rat lung tissue, Rat kidney tissue, Mouse heart tissue All lanes: ARHGAP18 antibody at 4.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 75, 71 kDa Observed band size: 75 kDa IHC image of CSB-PA850791LA01HU diluted at 1:200 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA850791LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ARHGAP18 Polyclonal antibody

Uniprot No.

Q8N392

Target Names

ARHGAP18

Alternative Names

4833419J07Rik antibody; AI314906 antibody; AI843501 antibody; Arhgap18 antibody; bA307O14.2 antibody; FLJ25728 antibody; MacGAP antibody; MGC126757 antibody; MGC138145 antibody; OTTHUMP00000017190 antibody; OTTMUSP00000028606 antibody; RHG18_HUMAN antibody; Rho GTPase-activating protein 18 antibody; Rho-type GTPase-activating protein 18 antibody; SENEX antibody

Raised in

Rabbit

Species Reactivity

Human, Rat, Mouse

Immunogen

Recombinant Human Rho GTPase-activating protein 18 protein (389-577AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ARHGAP18 Antibody (Product code: CSB-PA850791LA01HU) is Non-conjugated. For ARHGAP18 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA850791LB01HU	ARHGAP18 Antibody, HRP conjugated	ELISA
FITC	CSB-PA850791LC01HU	ARHGAP18 Antibody, FITC conjugated
Biotin	CSB-PA850791LD01HU	ARHGAP18 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Rho GTPase activating protein that suppresses F-actin polymerization by inhibiting Rho. Rho GTPase activating proteins act by converting Rho-type GTPases to an inactive GDP-bound state. Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts downstream of YAP1 and inhibits actin polymerization, which in turn reduces nuclear localization of YAP1. Regulates cell shape, spreading, and migration.

Gene References into Functions

Our data suggest that ARHGAP18, which was located by the SNP rs11759328 via positive selection, plays a potential role in regulating HbF expression in beta-thalassaemia and may be a promising therapeutic target PMID: 28983712
these results define opposing roles for oncogenic ARHGAP18 and tumor suppressive miR-200b in determining triple-negative breast cancer cell migration and metastatic prowess PMID: 28619708
data suggest the ARHGAP18 may confer vulnerability to SZ in the Chinese Han population, providing additional evidence for the involvement of neurodevelopmental dysfunction in the pathogenesis of schizophrenia PMID: 28384650
In endothelial cells, ARHGAP18 may act as a significant regulator of vascular homeostasis. PMID: 28251925
Our results show that miR-181a is down-regulated in glioblastoma multiforme (GBM) patients. The three target genes, ANGPT2, ARHGAP18 and LAMC1, are negatively correlated with the expression of miR-181a. Moreover, high expression of ANGPT2 or LAMC1 together with large size of GBM is correlated with a shorter median overall survival PMID: 27176932
Results describe ARHGAP18 as a novel negative regulator of sprouting by acting dualistically to limit tip cell formation and to maintain junctional integrity. PMID: 25425145
The results define ARHGAP18 as one of the crucial factors for the regulation of RhoA for the control of cell shape, spreading, and migration. PMID: 21865595
identification of a novel gene, SENEX, that regulates stress induced premature senescence pathways in endothelial cells involving p16(INK4a) and retinoblastoma protein activation PMID: 20664062
This study use Functional MRI and Genome Wide Association Analysis indentic novel gene(ARHGAP18) associated with schizophrenia. PMID: 19065146

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Subcellular Location

Cytoplasm.

Database Links

HGNC: 21035

OMIM: 613351

KEGG: hsa:93663

STRING: 9606.ENSP00000275189

UniGene: Hs.486458

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