ARID3A Antibody

Datasheet

Code	CSB-PA858728LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Mouse liver tissue All lanes: ARID3A antibody at 2.7μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 63 kDa Observed band size: 63 kDa IHC image of CSB-PA858728LA01HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA858728LA01HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA858728LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ARID3A Polyclonal antibody

Uniprot No.

Q99856

Target Names

ARID3A

Alternative Names

ARI3A_HUMAN antibody; ARID domain containing 3A antibody; ARID domain-containing protein 3A antibody; ARID3A antibody; AT rich interactive domain 3A (BRIGHT- like) protein antibody; AT rich interactive domain 3A (BRIGHT-like) antibody; AT rich interactive domain-containing protein 3A antibody; AT-rich interactive domain-containing protein 3A antibody; B cell regulator of IgH transcription antibody; B-cell regulator of IgH transcription antibody; Bright antibody; dead ringer like 1 antibody; dead ringer; Drosophila; homolog-like 1 antibody; Dead ringer-like protein 1 antibody; DRIL1 antibody; DRIL3 antibody; DRX antibody; E2F binding protein 1 antibody; E2F-binding protein 1 antibody; E2FBP1 antibody; Homo sapiens AT rich interactive domain 3A (BRIGHT-like) antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human AT-rich interactive domain-containing protein 3A protein (26-137AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ARID3A Antibody (Product code: CSB-PA858728LA01HU) is Non-conjugated. For ARID3A Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA858728LB01HU	ARID3A Antibody, HRP conjugated	ELISA
FITC	CSB-PA858728LC01HU	ARID3A Antibody, FITC conjugated
Biotin	CSB-PA858728LD01HU	ARID3A Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Transcription factor which may be involved in the control of cell cycle progression by the RB1/E2F1 pathway and in B-cell differentiation.

Gene References into Functions

Data indicate ARID3a(+) B cells as a type of effector B cell, and link ARID3a expression in B lymphocytes to interferon alpha (IFNa)-associated inflammatory responses in systemic lupus erythematosus (SLE). PMID: 27522115
results suggest that appropriate regulation of ARID3a is critical for normal development of both myeloid and B lineage pathways. PMID: 26685208
These data reveal new functions for ARID3a in early hematopoiesis and suggest that knowledge regarding ARID3a levels in HSPCs could be informative for applications requiring transplantation of those cells. PMID: 25535283
Systemic lupus erythematosus (SLE) patients had increased ARID3a+ B cells compared to healthy controls. ARID3a was not expressed in naive B cells of controls, but was abundant in SLE patients. Number of ARID3a+ B cells correlated with disease activity. PMID: 25185498
miR-125b can act as an oncogene in B-cell acute lymphoblastic leukemia by targeting ARID3a and mediating its repression. PMID: 22469780
These findings support the hypothesis that Epstein-Barr virus EBNA1 initiates transcription at the C promoter via interactions between multiple EBNA1 homodimers and cellular transcription such as E2F1, ARID3A and Oct-2. PMID: 22302879
These results indicate both cooperative and interdependent roles for ARID3A and p53 in the transcriptional activation of p21(WAF1) in response to DNA damage. PMID: 22172947
functions as a critical antagonist to the p16(INK4A)-Rb tumor suppressor machinery by regulating promyelocytic leukemia protein stability PMID: 22010578
report that E2FBP1 inhibits accumulation of ICP0 RNA and, at the same time, is degraded via ICP0's herpes ubiquitin ligase 2 (HUL-2) activity upon HSV-1 infection. PMID: 21248039
Bright/ARID3a inhibition causes increased developmental plasticity in mouse and human cells. PMID: 20680960
Solution NMR structure of the ARID domain of human ARID3A. PMID: 20455271
Results show that DRIL1 disrupts cellular protection against RAS(V12)-induced proliferation downstream of the p19(ARF)/p53 pathway. PMID: 11812999
role in p53 regulatory pathway.(E2FBP1) PMID: 12136662
Variations in Bright binding and matrix attachment region activity contribute to localized control of accessibility and therefore nonrandom gene use during V(D)J recombination. PMID: 12193717
a putative p53-binding site was found, which specifically responded to p53, in the second intron of the E2FBP1/DRIL1 gene PMID: 12692263
E2FBP1 modulates cell growth through down-regulation of promyelocytic leukemia bodies. PMID: 15017387
Bright is not expressed in all human B lymphocyte subpopulations. PMID: 15203319
TFII-I directly interacts with Bright through amino acids in Bright's protein interaction domain PMID: 16738337
identify Bright as a contributor to accessibility of the IgH enhancer PMID: 17386101
Id1 inhibited DNA binding by Dril1, and the two proteins co-localized in vitro and in vivo, providing a potential mechanism for suppression of fibrosis by Id1 through inhibition of the profibrotic function of Dril1. PMID: 18583319
A palmitoylated pool of Bright is diverted to lipid rafts of resting B cells where it associates with signalosome components. PMID: 19214191

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Subcellular Location

Nucleus. Cytoplasm. Note=Shuttles between nucleus and cytoplasm.

Tissue Specificity

Widely expressed, with highest expression in skeletal muscle, thalamus, and colon.

Database Links

HGNC: 3031

OMIM: 603265

KEGG: hsa:1820

STRING: 9606.ENSP00000263620

UniGene: Hs.501296

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