Product Details

Uniprot No.

Target Names

ASNA1

Alternative Names

ARSA 1 antibody; ARSA antibody; ArsA arsenite transporter ATP binding homolog 1 antibody; ArsA arsenite transporter; ATP binding; E. coli; homolog of; 1 antibody; ArsA arsenite transporter; ATP-binding; homolog 1 (bacterial) antibody; ARSA I antibody; ARSA-I antibody; ARSA1 antibody; ARSAI antibody; Arsenical pump driving ATPase antibody; Arsenical pump-driving ATPase antibody; Arsenical resistance ATPase antibody; Arsenite translocating ATPase antibody; Arsenite transporting ATPase antibody; Arsenite-stimulated ATPase antibody; ASNA 1 antibody; ASNA I antibody; ASNA-I antibody; ASNA_HUMAN antibody; ASNA1 antibody; Asna1 protein antibody; ASNAI antibody; ATPase ASNA1 antibody; GET3 antibody; Golgi to ER traffic 3 homolog antibody; hARSA-I antibody; hASNA-I antibody; MGC3821 antibody; Transmembrane domain recognition complex 40 kDa ATPase subunit antibody; Transmembrane domain recognition complex; 40kDa antibody; TRC40 antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Human ASNA1

Immunogen Species

Homo sapiens (Human)

Isotype

IgG

Purification Method

Antigen Affinity purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.

Tested Applications

ELISA,WB,IHC,IF

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1/WRB and CAMLG/GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-CAMLG receptor, and returning it to the cytosol to initiate a new round of targeting. May be involved in insulin signaling.

Gene References into Functions

The authors conclude that the TRC40 pathway is critical for hearing and propose that otoferlin is an essential substrate of this pathway in hair cells. PMID: 27458190
Asna1/TRC40 is required at a late step of herpes simplex virus type 1 infection for efficient release of mature virions to the extracellular milieu. PMID: 27765046
reveal unanticipated complexity in the mutual regulation of the TRC40 receptor subunits and raise the question as to the role of the excess CAML in the endoplasmic reticulum PMID: 27226539
Emerin interacts with TRC40 in situ. PMID: 26675233
The repertoire of VAPB interactors is more diverse than previously anticipated and link VAPB to the function of ATPase complexes such as p97/FAF1 and ASNA1/transmembrane-domain recognition complex. PMID: 24885147
Proteins bind to TRC40 and can utilise this component for their delivery to the ER membrane. PMID: 22505607
PEX19 formed a complex with the peroxisomal tail anchored protein PEX26 in the cytosol and translocated it directly to peroxisomes by a TRC40-independent class I pathway. PMID: 23460677
Results indicate calcium-modulating cyclophilin ligand (CAML) and WRB as components of the TRC40 receptor complex and a crucial mechanism for driving ER membrane insertion of TA proteins in mammalian cells. PMID: 23041287
Post-translational membrane insertion of tail-anchored transmembrane EF-hand Ca2+ sensor calneurons requires the TRC40/Asna1 protein chaperone. PMID: 21878631
The coiled-coil domain of WRB is the binding site for TRC40/Asna1. PMID: 21444755
Asna1 is required for efficient histocompatibility (HLA) class I downregulation mediated by viral protein BNLF2a. PMID: 21296983
Asna1 can mediate membrane insertion of RAMP4 and Sec61beta without the participation of other cytosolic proteins by a mechanism that depends on the presence of ATP or ADP and a protease-sensitive receptor in the endoplasmic reticulum membrane. PMID: 20375064
Human ASNA1 is highly expressed in pancreatic beta cells, but not in other pancreatic endocrine cell types, and regulates insulin secretion in cultured cells. PMID: 17289575
TRC40/Asna-1 interacts posttranslationally with tail-anchored proteins in a transmembrane domain-dependent manner for delivery to a proteinaceous receptor at the endoplasmic reticulum membrane. PMID: 17382883
the hydrophobicity of the TA region dictates whether a precursor is delivered to the ER via the Hsp40/Hsc70 or Asna-1/TRC40-dependent route. PMID: 18667436
ASNA1 is a target to overcome platinum resistance in ovarian cancer PMID: 19724867

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Subcellular Location

Cytoplasm. Endoplasmic reticulum. Nucleus, nucleolus.

Protein Families

ArsA ATPase family

Tissue Specificity

Expressed in the epithelial cells of the liver, kidney, and stomach wall, in the adrenal medulla, in the islet cells of the pancreas, in the red pulp of the spleen, and in cardiac and skeletal muscle.

Database Links

HGNC: 752

OMIM: 601913

KEGG: hsa:439

STRING: 9606.ENSP00000349887

UniGene: Hs.465985

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Code	CSB-PA002218GA01HU
Size	$600
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ASNA1 Antibody

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Target Background