ATP2C2 Antibody

Datasheet

Code	CSB-PA002340LA01HU
Size	US$166
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Image	Immunofluorescence staining of A549 cells with CSB-PA002340LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ATP2C2 Polyclonal antibody

Uniprot No.

O75185

Target Names

ATP2C2

Alternative Names

AT2C2_HUMAN antibody; ATP2C2 antibody; ATPase 2C2 antibody; ATPase; Ca++ transporting; type 2C; member 2 antibody; Calcium-transporting ATPase type 2C member 2 antibody; DKFZp686H22230 antibody; hSPCA2 antibody; KIAA0703 antibody; PMR1 antibody; Secretory pathway Ca(2+)-ATPase 2 antibody; secretory pathway calcium ATPase 2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Calcium-transporting ATPase type 2C member 2 protein (621-702AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ATP2C2 Antibody (Product code: CSB-PA002340LA01HU) is Non-conjugated. For ATP2C2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA002340LB01HU	ATP2C2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA002340LC01HU	ATP2C2 Antibody, FITC conjugated
Biotin	CSB-PA002340LD01HU	ATP2C2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IF

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

ATP-driven pump that supplies the Golgi apparatus with Ca(2+) and Mn(2+) ions, both essential cofactors for processing and trafficking of newly synthesized proteins in the secretory pathway. Within a catalytic cycle, acquires Ca(2+) or Mn(2+) ions on the cytoplasmic side of the membrane and delivers them to the lumenal side. The transfer of ions across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing to outward-facing state. Induces Ca(2+) influx independently of its ATP-driven pump function. At the basolateral membrane of mammary epithelial cells, interacts with Ca(2+) channel ORAI1 and mediates Ca(2+) entry independently of the Ca(2+) content of endoplasmic reticulum or Golgi stores. May facilitate transepithelial transport of large quantities of Ca(2+) for milk secretion via activation of Ca(2+) influx channels at the plasma membrane and active Ca(2+) transport at the Golgi apparatus.

Gene References into Functions

In total, four independent SNPs within DYX1C1 and ATP2C2 were found to be associated with MMR stronger than expected from multiple testing. To explore potential pathomechanisms, we annotated these variants with functional data including tissue-specific expression analysis and eQTLs. PMID: 29201552
The Secretory Pathway Ca(2+) -ATPases SPCA1 and SPCA2 are strongly induced under osteogenic conditions that elicit microcalcifications. SPCA gene expression is significantly elevated in breast cancer subtypes that are associated with microcalcifications. PMID: 28618103
The extract was found to comprise mainly primary fatty acid amides (PFAAs) by NMR analysis. Individual PFAAs, especially oleamide and linoleamide, almost completely inhibited hSPCA2 activity with IC50 values of 7.5 muM and 3.8 muM, respectively. PMID: 27297103
we found that SPCA2-transfected cells display a higher number of cells entering S phase. Altogether, our results point at the important role of SPCA2 in regulation of cell cycle in cancer cells PMID: 27316461
Orai1-mediated Ca(2+)-influx and SPCA2-mediated Ca(2+) uptake activity into the Golgi/secretory pathway might be coupled possibly in a microdomain. This channel/pump complex may efficiently transfer Ca(2+) into the secretory pathway, which might play a role in SPCA2-expressing secretory cells, such as mammary gland during lactation. PMID: 27692665
Supporting a more general neurocognition role of ATP2C2 is the previous association of ATP2C2 with attention deficit hyperactivity disorder, a condition commonly comorbid with dyslexia and language impairment. PMID: 25448322
Findings reveal a signaling pathway in which the Orai1-SPCA2 complex elicits constitutive store-independent Ca(2+) signaling that promotes tumorigenesis. PMID: 20887894
SPCA2 may have a more specialized role in mammalian cells, possibly in cellular detoxification of Mn2+ ions. PMID: 15677451
hSPCA2 has the ability to transport Ca(2+) and Mn(2+); both its transport and Ca(2+)- and Mn(2+)-dependent phosphoprotein intermediate formation functions are insensitive to thapsigargin inhibition PMID: 15831496
analysis of SPCA1 and SPCA2 isoenzymes by steady-state and transient kinetic analyses PMID: 16332677
ATP2C2 modulates phonological short-term memory in language impairment. PMID: 19646677

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Subcellular Location

Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein.

Protein Families

Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIA subfamily

Tissue Specificity

Highly expressed in the gastrointestinal and respiratory tracts, prostate, thyroid, salivary, and mammary glands. Expressed in colon epithelial cells (at protein level). Expressed in brain and testis (at protein level).

Database Links

HGNC: 29103

OMIM: 613082

KEGG: hsa:9914

STRING: 9606.ENSP00000262429

UniGene: Hs.6168

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