ATP6V0D2 Antibody

Code CSB-PA818735LA01HU
Size US$166
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Image
  • Western Blot
    Positive WB detected in: A549 whole cell lysate, PC3 whole cell lysate
    All lanes: ATP6V0D2 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 41 kDa
    Observed band size: 41 kDa

  • IHC image of CSB-PA818735LA01HU diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-PA818735LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) ATP6V0D2 Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
ATP6V0D2V-type proton ATPase subunit d 2 antibody; V-ATPase subunit d 2 antibody; Vacuolar proton pump subunit d 2 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human V-type proton ATPase subunit d 2 protein (50-295AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The ATP6V0D2 Antibody (Product code: CSB-PA818735LA01HU) is Non-conjugated. For ATP6V0D2 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA818735LB01HU ATP6V0D2 Antibody, HRP conjugated ELISA
FITC CSB-PA818735LC01HU ATP6V0D2 Antibody, FITC conjugated
Biotin CSB-PA818735LD01HU ATP6V0D2 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:500-1:1000
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis.
Gene References into Functions
  1. gammadelta T cells suppressed iDCs osteoclastogenesis by downregulation of the RANK/cFos/ATP6V0D2 signaling pathway. PMID: 30066839
  2. The secondary structures of d1 and d2 subunits were highly similar, but the relative stability against thermal stress was higher for d1 than d2. PMID: 24631925
  3. these data suggest that Adrm1, a new Atp6v0d2-interacting protein, plays an important role in osteoclast differentiation, and in particular the fusion of preosteoclasts. PMID: 19818731
Protein Families
V-ATPase V0D/AC39 subunit family
Tissue Specificity
Kidney, osteoclast and lung.
Database Links

HGNC: 18266

KEGG: hsa:245972

STRING: 9606.ENSP00000285393

UniGene: Hs.436360

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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