ATP6V0D2 Antibody

Datasheet

Code	CSB-PA818735LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: A549 whole cell lysate, PC3 whole cell lysate All lanes: ATP6V0D2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41 kDa Observed band size: 41 kDa IHC image of CSB-PA818735LA01HU diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA818735LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ATP6V0D2 Polyclonal antibody

Uniprot No.

Q8N8Y2

Target Names

ATP6V0D2

Alternative Names

ATP6V0D2V-type proton ATPase subunit d 2 antibody; V-ATPase subunit d 2 antibody; Vacuolar proton pump subunit d 2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human V-type proton ATPase subunit d 2 protein (50-295AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ATP6V0D2 Antibody (Product code: CSB-PA818735LA01HU) is Non-conjugated. For ATP6V0D2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA818735LB01HU	ATP6V0D2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA818735LC01HU	ATP6V0D2 Antibody, FITC conjugated
Biotin	CSB-PA818735LD01HU	ATP6V0D2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis.

Gene References into Functions

gammadelta T cells suppressed iDCs osteoclastogenesis by downregulation of the RANK/cFos/ATP6V0D2 signaling pathway. PMID: 30066839
The secondary structures of d1 and d2 subunits were highly similar, but the relative stability against thermal stress was higher for d1 than d2. PMID: 24631925
these data suggest that Adrm1, a new Atp6v0d2-interacting protein, plays an important role in osteoclast differentiation, and in particular the fusion of preosteoclasts. PMID: 19818731

Protein Families

V-ATPase V0D/AC39 subunit family

Tissue Specificity

Kidney, osteoclast and lung.

Database Links

HGNC: 18266

KEGG: hsa:245972

STRING: 9606.ENSP00000285393

UniGene: Hs.436360

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Oxidative Phosphorylation Synaptic vesicle cycle

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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