AUP1 Antibody

Datasheet

Code	CSB-PA951577
Size	US$166
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Image	The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA951577(AUP1 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA951577(AUP1 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200) Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Hela cells, 293T cells, Primary antibody: CSB-PA951577(AUP1 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

Q9Y679

Target Names

AUP1

Alternative Names

AA589454 antibody; Ancient ubiquitous protein 1 antibody; AUP 1 antibody; aup1 antibody; AUP1_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein of Human AUP1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form

Liquid

Tested Applications

ELISA,WB,IHC

Recommended Dilution

Application	Recommended Dilution
ELISA	1:2000-1:5000
WB	1:500-1:2000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Plays a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. Plays a role in lipid droplet formation. Induces lipid droplet clustering. Recruits ubiquitin-conjugating enzyme UBE2G2 to lipid droplets which facilitates its interaction with ubiquitin ligases AMFR/gp78 and RNF139/TRC8, leading to sterol-induced ubiquitination of HMGCR and its subsequent proteasomal degradation. Also required for the degradation of INSIG1, SREBF1 and SREBF2. Plays a role in regulating assembly and secretion of very low density lipoprotein particles and stability of apolipoprotein APOB.; (Microbial infection) Following Dengue virus infection, required for induction of lipophagy which facilitates production of virus progeny particles.

Gene References into Functions

AUP1 may be a crucial factor in hepatic apoB100 quality control, determining the rate at which apoB100 is degraded or lipidated to enable VLDL particle assembly and secretion. PMID: 28183703
Monoubiquitinated AUP1 on the lipid droplet surface specifically induces clustering. PMID: 24039768
A novel role for Aup1 in maintenance of intracellular cholesterol homeostasis via mediation of the endoplasmic reticulum associated degradation of HMG-CoA reductase. PMID: 23223569
AUP1 targeting signals PMID: 23197321
Dual role of ancient ubiquitous protein 1 (AUP1) in lipid droplet accumulation and endoplasmic reticulum (ER) protein quality control. PMID: 21857022
The presence of the AUP1-Ube2g2 complex at LDs provides a direct molecular link between LDs and the cellular ubiquitination machinery. PMID: 21127063
binding of Aup1 plays a crucial role in the alpha(IIb)beta(3) inside-out signaling PMID: 12042322
molecular cloning and sequencing of cDNA and preliminary characterization of protein PMID: 12534237

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Subcellular Location

Endoplasmic reticulum membrane; Peripheral membrane protein. Lipid droplet.; Cytoplasmic vesicle, autophagosome.

Protein Families

AUP1 family

Tissue Specificity

Detected in blood platelets and leukocytes (at protein level). Ubiquitous. Highly expressed in placenta, liver, kidney, skeletal muscle, heart and brain.

Database Links

HGNC: 891

OMIM: 602434

KEGG: hsa:550

STRING: 9606.ENSP00000366748

UniGene: Hs.411480

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Phage Display Service

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Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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