Acetyl-HIST1H2BC (K11) Antibody

^Datasheet

Code	CSB-PA010403OA11acHU
Size	US$319
Image	Western Blot Detected samples: 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h All lanes: HIST1H2BC antibody at 1:100 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa Immunocytochemistry analysis of CSB-PA010403OA11acHU diluted at 1:20 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Chromatin Immunoprecipitation Hela (10⁶, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2BC (CSB-PA010403OA11acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

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Target Data

Product Details

Full Product Name

Rabbit anti-Human HIST1H2BC Polyclonal antibody

Uniprot No.

P62807

Target Names

HIST1H2BC

Alternative Names

Histone H2B type 1-C/E/F/G/I (Histone H2B.1 A) (Histone H2B.a) (H2B/a) (Histone H2B.g) (H2B/g) (Histone H2B.h) (H2B/h) (Histone H2B.k) (H2B/k) (Histone H2B.l) (H2B/l), HIST1H2BC; HIST1H2BE; HIST1H2BF; HIST1H2BG; HIST1H2BI, H2BFL; H2BFH; H2BFG; H2BFA; H2BFK

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Peptide sequence around site of Acetyl-Lys (11) derived from Human Histone H2B type 1-C/E/F/G/I

Immunogen Species

Human

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, ChIP

Recommended Dilution

Application	Recommended Dilution
WB	1:100-1:1000
IHC	1:10-1:100

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.; FUNCTION
Subcellular Location	Nucleus, Chromosome
Protein Families	Histone H2B family
Database Links	HGNC: 4757 OMIM: 602798 KEGG: hsa:3017 STRING: 9606.ENSP00000366962 UniGene: Hs.182137