B3GNT6 Antibody

Datasheet

Code	CSB-PA744392LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: K562 whole cell lysate, HT29 whole cell lysate All lanes: B3GNT6 antibody at 10µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43, 30 kDa Observed band size: 43 kDa IHC image of CSB-PA744392LA01HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
Citations
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Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) B3GNT6 Polyclonal antibody

Uniprot No.

Q6ZMB0

Target Names

B3GNT6

Alternative Names

B3GNT6Acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase antibody; EC 2.4.1.147 antibody; Core 3 synthase antibody; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6 antibody; BGnT-6 antibody; Beta-1,3-Gn-T6 antibody; Beta-1,3-N-acetylglucosaminyltransferase 6 antibody; Beta3Gn-T6 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase protein (251-345AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The B3GNT6 Antibody (Product code: CSB-PA744392LA01HU) is Non-conjugated. For B3GNT6 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA744392LB01HU	B3GNT6 Antibody, HRP conjugated	ELISA
FITC	CSB-PA744392LC01HU	B3GNT6 Antibody, FITC conjugated
Biotin	CSB-PA744392LD01HU	B3GNT6 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Restoring O-glycosylation and expression of MUC2 limits progression of colorectal cancer A Kang,/,2024

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : Co-immunoprecipitation (Co-IP)

Sample type: cells

Review: Furthermore, immunoprecipitation experiments on LOVO cells transfected with the pcDNA3.1-B3GNT6 vector showed that B3GNT6 can directly interact with MUC2 (Figure 2.G). The BSL-II lectin staining demonstrated that overexpression of B3GNT6 caused a significant increase in the level of Core3 O-glycosylation in MUC2 (Figure 2.H).

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Target Background

Function

Beta-1,3-N-acetylglucosaminyltransferase that synthesizes the core 3 structure of the O-glycan, an important precursor in the biosynthesis of mucin-type glycoproteins. Plays an important role in the synthesis of mucin-type O-glycans in digestive organs.

Gene References into Functions

expression was markedly down-regulated in gastric and colorectal carcinomas PMID: 15755813
alpha2beta1 integrin acquired core3 O-glycans in cells expressing core3 synthase with decreased maturation of beta1 integrin, leading to decreased levels of the alpha2beta1 integrin complex PMID: 19395705

Subcellular Location

Golgi apparatus membrane; Single-pass type II membrane protein.

Protein Families

Glycosyltransferase 31 family

Tissue Specificity

Present in stomach and colon (at protein level). Restricted in the stomach, colon and small intestine, where core 3 structure is present.

Database Links

HGNC: 24141

OMIM: 615315

KEGG: hsa:192134

STRING: 9606.ENSP00000435352

UniGene: Hs.352622

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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