BARHL1 Antibody

Datasheet
Code CSB-PA880147LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. Q9BZE3
Image
  • Western Blot
    Positive WB detected in: SH-SY5Y whole cell lysate, U251 whole cell lysate
    All lanes: BARHL1 antibody at 3.4µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 36 kDa
    Observed band size: 36 kDa

  • IHC image of CSB-PA880147LA01HU diluted at 1:400 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunogen Recombinant Human BarH-like 1 homeobox protein (38-176AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500
Relevance nucleus, RNA polymerase II regulatory region sequence-specific DNA binding, transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding, regulation of transcription from RNA polymerase II promoter
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias BarH-like 1 homeobox protein, BARHL1
Immunogen Species Human
Research Area Others
Target Names BARHL1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Subcellular Location Nucleus
Protein Families BAR homeobox family
Database Links

HGNC: 953

OMIM: 605211

KEGG: hsa:56751

STRING: 9606.ENSP00000263610

UniGene: Hs.283809

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