CARD6 Antibody

Datasheet

Code	CSB-PA863138LA01HU
Size	US$166
	Order now
Image	IHC image of CSB-PA863138LA01HU diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of U251 cells with CSB-PA863138LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Promotion
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CARD6 Polyclonal antibody

Uniprot No.

Q9BX69

Target Names

CARD6

Alternative Names

CARD 6 antibody; CARD containing inhibitor of Nod1 and Cardiak-induced NF kB activation antibody; CARD6 antibody; CARD6_HUMAN antibody; Caspase recruitment domain family; member 6 antibody; Caspase recruitment domain protein 6 antibody; Caspase recruitment domain-containing protein 6 antibody; CINCIN1 antibody; D730008L15 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Caspase recruitment domain-containing protein 6 protein (666-930AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CARD6 Antibody (Product code: CSB-PA863138LA01HU) is Non-conjugated. For CARD6 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA863138LB01HU	CARD6 Antibody, HRP conjugated	ELISA
FITC	CSB-PA863138LC01HU	CARD6 Antibody, FITC conjugated
Biotin	CSB-PA863138LD01HU	CARD6 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

May be involved in apoptosis.

Gene References into Functions

The increased expression of CARD6 in esophageal squamous cell carcinoma,gastric and colorectal adenocarcinoma suggested that neoexpression of CARD6 might be related to activation of NF-kappaB pathway. PMID: 20025480
identification as a modulator of NF-kappa B activation by Nod1- and Cardiak-mediated pathways PMID: 12775719
CARD6 is a regulator of NF-kappaB activation that modulates the functions of RICK protein PMID: 16418290
review of the regulation of interactions of CARD6 with RICK and microtubules [review] PMID: 16582588

Database Links

HGNC: 16394

OMIM: 609986

KEGG: hsa:84674

STRING: 9606.ENSP00000254691

UniGene: Hs.200242

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

NOD-like receptor signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
If CAPTCHA verification fails, click to refresh the CAPTCHA and try again.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address