CARD6 Antibody

Code CSB-PA863138LA01HU
Size US$166
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Image
  • IHC image of CSB-PA863138LA01HU diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of U251 cells with CSB-PA863138LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) CARD6 Polyclonal antibody
Uniprot No.
Target Names
CARD6
Alternative Names
CARD 6 antibody; CARD containing inhibitor of Nod1 and Cardiak-induced NF kB activation antibody; CARD6 antibody; CARD6_HUMAN antibody; Caspase recruitment domain family; member 6 antibody; Caspase recruitment domain protein 6 antibody; Caspase recruitment domain-containing protein 6 antibody; CINCIN1 antibody; D730008L15 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Caspase recruitment domain-containing protein 6 protein (666-930AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The CARD6 Antibody (Product code: CSB-PA863138LA01HU) is Non-conjugated. For CARD6 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA863138LB01HU CARD6 Antibody, HRP conjugated ELISA
FITC CSB-PA863138LC01HU CARD6 Antibody, FITC conjugated
Biotin CSB-PA863138LD01HU CARD6 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:200-1:500
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
May be involved in apoptosis.
Gene References into Functions
  1. The increased expression of CARD6 in esophageal squamous cell carcinoma,gastric and colorectal adenocarcinoma suggested that neoexpression of CARD6 might be related to activation of NF-kappaB pathway. PMID: 20025480
  2. identification as a modulator of NF-kappa B activation by Nod1- and Cardiak-mediated pathways PMID: 12775719
  3. CARD6 is a regulator of NF-kappaB activation that modulates the functions of RICK protein PMID: 16418290
  4. review of the regulation of interactions of CARD6 with RICK and microtubules [review] PMID: 16582588
Database Links

HGNC: 16394

OMIM: 609986

KEGG: hsa:84674

STRING: 9606.ENSP00000254691

UniGene: Hs.200242

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