Tel : 301-363-4651
All
  • All
  • Kit
  • Protein
  • Antibody
  • Molecular Biology Product
  • Small Molecule
  • Raw Material For IVD Kit

CLDN18.2｜CD47｜Proteinase K｜EGFR｜PD-L1｜CD276｜ACE2｜TMPRSS2｜Exosome Isolation Kits

SARS-CoV-2: Nucleoprotein｜Spike protein｜S (RBD) protein｜N Antibody｜S Antibody｜Neutralizing Ab ELISA Kit

CCNY Antibody

Datasheet
Code CSB-PA847689LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image

  • Western Blot
    Positive WB detected in: K562 whole cell lysate
    All lanes: CCNY antibody at 6µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 40, 37, 34 kDa
    Observed band size: 40 kDa

  • IHC image of CSB-PA847689LA01HU diluted at 1:600 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-PA847689LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

The Latest Promotion Free Antibody trial simple
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) CCNY Polyclonal antibody
Uniprot No. Q8ND76
Target Names CCNY
Alternative Names CCNY antibody; C10orf9 antibody; CBCP1 antibody; CFP1Cyclin-Y antibody; Cyc-Y antibody; Cyclin box protein 1 antibody; Cyclin fold protein 1 antibody; cyclin-X antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Cyclin-Y protein (2-170AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:500-1:1000
IF 1:200-1:500
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Related Products

CCNY antibodies

CCNY Proteins

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Data

Function Positive regulatory subunit of the cyclin-dependent kinases CDK14/PFTK1 and CDK16. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma membrane and promoting phosphorylation of LRP6, leading to the activation of the Wnt signaling pathway. Recruits CDK16 to the plasma membrane. Isoform 3 might play a role in the activation of MYC-mediated transcription.
Gene References into Functions
  1. increased expression of CCNY was significantly associated with cell proliferation and migration. PMID: 29557391
  2. Amplification of gene CCNY is associated with metastatic lung adenocarcinoma. PMID: 28381877
  3. we believe that CCNY has biological effect in breast cancer (BC) development, and its inhibition via an RNA interference lentiviral system may provide a therapeutic option for BC PMID: 27666310
  4. CCNY was significantly upregulated in ovarian cancer cell lines and tissues. Significant association was observed between CCNY expression and clinicopathological stage and lymph node metastasis. It significantly exacerbated proliferation, migration, and invasion of A2780 cells, in vitro and in nude mice. It significantly increased c-Myc, cyclin D1, PFTK1, ki67, and OGT protein expressions in xenograft tumor tissues. PMID: 26831658
  5. Activation of PCTAIRE-1 is mediated through interaction with the phosphorylated form of cyclin Y in complex with 14-3-3. PMID: 26205494
  6. Our findings highlight a PFTK1-CCNY complex in activating noncanonical Wnt signaling in HCC cells. PMID: 24824184
  7. Human cyclin Y (CCNY) is a phosphoprotein in vivo and phosphorylation of CCNY by CDK14 triggers its ubiquitination and degradation. PMID: 24794231
  8. Cyclin Y regulates the expression and membrane trafficing of PCTAIRE kinases (PCTK). (Review) PMID: 22895054
  9. CCNY binding to CDK16 required a region upstream of the kinase domain and was found to be inhibited by phosphorylation of serine 153, a potential PKA phosphorylation site. PMID: 22184064
  10. these results validated the role of CCNY as a clinically relevant human oncoprotein PMID: 21273179
  11. CCNY might play an important role in glioma tumorigenisis. PMID: 20441050
  12. Single nucleotide polymorphism in CCNY gene is associated with Crohn's disease and ulcerative colitis PMID: 18438405

Show More

Hide All
Subcellular Location Cell membrane, Lipid-anchor, Cytoplasmic side, SUBCELLULAR LOCATION: Isoform 3: Nucleus
Protein Families Cyclin family, Cyclin Y subfamily
Tissue Specificity Widely expressed.
Database Links

HGNC: 23354

OMIM: 612786

KEGG: hsa:219771

STRING: 9606.ENSP00000363836

UniGene: Hs.14745
Promotions
Contact Us

  Tel: 301-363-4651
  Email: [email protected]
  Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Related Articles
Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1