CCNY Antibody

Datasheet

Code	CSB-PA847689LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: K562 whole cell lysate All lanes: CCNY antibody at 6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40, 37, 34 kDa Observed band size: 40 kDa IHC image of CSB-PA847689LA01HU diluted at 1:600 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA847689LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CCNY Polyclonal antibody

Uniprot No.

Q8ND76

Target Names

CCNY

Alternative Names

CCNY antibody; C10orf9 antibody; CBCP1 antibody; CFP1Cyclin-Y antibody; Cyc-Y antibody; Cyclin box protein 1 antibody; Cyclin fold protein 1 antibody; cyclin-X antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Cyclin-Y protein (2-170AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CCNY Antibody (Product code: CSB-PA847689LA01HU) is Non-conjugated. For CCNY Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA847689LB01HU	CCNY Antibody, HRP conjugated	ELISA
FITC	CSB-PA847689LC01HU	CCNY Antibody, FITC conjugated
Biotin	CSB-PA847689LD01HU	CCNY Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Positive regulatory subunit of the cyclin-dependent kinases CDK14/PFTK1 and CDK16. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma membrane and promoting phosphorylation of LRP6, leading to the activation of the Wnt signaling pathway. Recruits CDK16 to the plasma membrane. Isoform 3 might play a role in the activation of MYC-mediated transcription.

Gene References into Functions

increased expression of CCNY was significantly associated with cell proliferation and migration. PMID: 29557391
Amplification of gene CCNY is associated with metastatic lung adenocarcinoma. PMID: 28381877
we believe that CCNY has biological effect in breast cancer (BC) development, and its inhibition via an RNA interference lentiviral system may provide a therapeutic option for BC PMID: 27666310
CCNY was significantly upregulated in ovarian cancer cell lines and tissues. Significant association was observed between CCNY expression and clinicopathological stage and lymph node metastasis. It significantly exacerbated proliferation, migration, and invasion of A2780 cells, in vitro and in nude mice. It significantly increased c-Myc, cyclin D1, PFTK1, ki67, and OGT protein expressions in xenograft tumor tissues. PMID: 26831658
Activation of PCTAIRE-1 is mediated through interaction with the phosphorylated form of cyclin Y in complex with 14-3-3. PMID: 26205494
Our findings highlight a PFTK1-CCNY complex in activating noncanonical Wnt signaling in HCC cells. PMID: 24824184
Human cyclin Y (CCNY) is a phosphoprotein in vivo and phosphorylation of CCNY by CDK14 triggers its ubiquitination and degradation. PMID: 24794231
Cyclin Y regulates the expression and membrane trafficing of PCTAIRE kinases (PCTK). (Review) PMID: 22895054
CCNY binding to CDK16 required a region upstream of the kinase domain and was found to be inhibited by phosphorylation of serine 153, a potential PKA phosphorylation site. PMID: 22184064
these results validated the role of CCNY as a clinically relevant human oncoprotein PMID: 21273179
CCNY might play an important role in glioma tumorigenisis. PMID: 20441050
Single nucleotide polymorphism in CCNY gene is associated with Crohn's disease and ulcerative colitis PMID: 18438405

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Subcellular Location

Cell membrane; Lipid-anchor; Cytoplasmic side.; [Isoform 3]: Nucleus.

Protein Families

Cyclin family, Cyclin Y subfamily

Tissue Specificity

Widely expressed.

Database Links

HGNC: 23354

OMIM: 612786

KEGG: hsa:219771

STRING: 9606.ENSP00000363836

UniGene: Hs.14745

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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