CD300LG Antibody

Code CSB-PA751016LA01HU
Size US$166
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Image
  • Western Blot
    Positive WB detected in: Rat kidney tissue
    All lanes: CD300LG antibody at 6μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 37, 27, 25, 34, 30 kDa
    Observed band size: 37 kDa

  • Immunofluorescence staining of 293 cells with CSB-PA751016LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC image of CSB-PA751016LA01HU diluted at 1:200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) CD300LG Polyclonal antibody
Uniprot No.
Target Names
CD300LG
Alternative Names
CD300 antigen-like family member G antibody; CD300 molecule like family member g antibody; CD300g antibody; Cd300lg antibody; CLM-9 antibody; CLM9 antibody; CLM9_HUMAN antibody; CMRF35-like molecule 9 antibody; Nepmucin antibody; TREM-4 antibody; TREM4 antibody; Triggering receptor expressed on myeloid cells 4 antibody
Raised in
Rabbit
Species Reactivity
Human, Rat
Immunogen
Recombinant Human CMRF35-like molecule 9 protein (128-241AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The CD300LG Antibody (Product code: CSB-PA751016LA01HU) is Non-conjugated. For CD300LG Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA751016LB01HU CD300LG Antibody, HRP conjugated ELISA
FITC CSB-PA751016LC01HU CD300LG Antibody, FITC conjugated
Biotin CSB-PA751016LD01HU CD300LG Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:200-1:500
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Receptor which may mediate L-selectin-dependent lymphocyte rollings. Binds SELL in a calcium dependent manner. Binds lymphocyte.
Gene References into Functions
  1. Both mRNA and protein levels of CD300LG-g in pulmonary carcinoma tissues were significantly lower than that in tumor-adjacent tissues, which might lead to inhibition of killing function of immunocytes, resulting in immune escape of lung cancer cells. PMID: 26977771
  2. Blood pressure, measured with a sensitive method, in a small sample of CD300LG rs72836561 CT-carriers was higher than in CC-carriers. PMID: 25314291
Subcellular Location
Apical cell membrane; Single-pass type I membrane protein. Basolateral cell membrane; Single-pass type I membrane protein. Endosome, multivesicular body membrane; Single-pass type I membrane protein.
Protein Families
CD300 family
Tissue Specificity
Highly expressed in heart, skeletal muscle and placenta.
Database Links

HGNC: 30455

OMIM: 610520

KEGG: hsa:146894

STRING: 9606.ENSP00000321005

UniGene: Hs.147313

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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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