CD300LG Antibody

Datasheet

Code	CSB-PA751016LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Rat kidney tissue All lanes: CD300LG antibody at 6μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 37, 27, 25, 34, 30 kDa Observed band size: 37 kDa Immunofluorescence staining of 293 cells with CSB-PA751016LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). IHC image of CSB-PA751016LA01HU diluted at 1:200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CD300LG Polyclonal antibody

Uniprot No.

Q6UXG3

Target Names

CD300LG

Alternative Names

CD300 antigen-like family member G antibody; CD300 molecule like family member g antibody; CD300g antibody; Cd300lg antibody; CLM-9 antibody; CLM9 antibody; CLM9_HUMAN antibody; CMRF35-like molecule 9 antibody; Nepmucin antibody; TREM-4 antibody; TREM4 antibody; Triggering receptor expressed on myeloid cells 4 antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human CMRF35-like molecule 9 protein (128-241AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CD300LG Antibody (Product code: CSB-PA751016LA01HU) is Non-conjugated. For CD300LG Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA751016LB01HU	CD300LG Antibody, HRP conjugated	ELISA
FITC	CSB-PA751016LC01HU	CD300LG Antibody, FITC conjugated
Biotin	CSB-PA751016LD01HU	CD300LG Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Receptor which may mediate L-selectin-dependent lymphocyte rollings. Binds SELL in a calcium dependent manner. Binds lymphocyte.

Gene References into Functions

Both mRNA and protein levels of CD300LG-g in pulmonary carcinoma tissues were significantly lower than that in tumor-adjacent tissues, which might lead to inhibition of killing function of immunocytes, resulting in immune escape of lung cancer cells. PMID: 26977771
Blood pressure, measured with a sensitive method, in a small sample of CD300LG rs72836561 CT-carriers was higher than in CC-carriers. PMID: 25314291

Subcellular Location

Apical cell membrane; Single-pass type I membrane protein. Basolateral cell membrane; Single-pass type I membrane protein. Endosome, multivesicular body membrane; Single-pass type I membrane protein.

Protein Families

CD300 family

Tissue Specificity

Highly expressed in heart, skeletal muscle and placenta.

Database Links

HGNC: 30455

OMIM: 610520

KEGG: hsa:146894

STRING: 9606.ENSP00000321005

UniGene: Hs.147313

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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