CD3EAP Antibody

Datasheet

Citations (1)

Code	CSB-PA004932LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, U87 whole cell lysate, Rat brain tissue All lanes: CD3EAP antibody at 3.9μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 55, 56 kDa Observed band size: 55, 56 kDa IHC image of CSB-PA004932LA01HU diluted at 1:200 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA004932LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
Citations
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Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CD3EAP Polyclonal antibody

Uniprot No.

O15446

Target Names

CD3EAP

Alternative Names

A34.5 antibody; Anti sense to ERCC 1 protein antibody; Antisense to ERCC-1 protein antibody; ASE 1 antibody; ASE-1 antibody; ASE1 antibody; CAST antibody; CD3 epsilon associated protein antibody; CD3-epsilon-associated protein antibody; CD3E antigen, epsilon polypeptide associated protein antibody; CD3E associated protein antibody; CD3e molecule, epsilon associated protein antibody; CD3E-associated protein antibody; CD3EAP antibody; DNA directed RNA polymerase I subunit RPA34 antibody; DNA-directed RNA polymerase I subunit RPA34 antibody; MGC118851 antibody; PAF 49 antibody; PAF49 antibody; RNA polymerase I associated factor PAF49 antibody; RNA polymerase I-associated factor PAF49 antibody; RPA34_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human DNA-directed RNA polymerase I subunit RPA34 protein (289-404AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CD3EAP Antibody (Product code: CSB-PA004932LA01HU) is Non-conjugated. For CD3EAP Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA004932LB01HU	CD3EAP Antibody, HRP conjugated	ELISA
FITC	CSB-PA004932LC01HU	CD3EAP Antibody, FITC conjugated
Biotin	CSB-PA004932LD01HU	CD3EAP Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

STAT3 potentiates RNA polymerase I-directed transcription and tumor growth by activating RPA34 expression C Zhang,British journal of cancer,2023

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Target Background

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.; Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.

Gene References into Functions

Haplotypes consisting of PPP1R13L, CD3EAP and GLTSCR1 SNPs on Chr19q13.3 may associate with lung cancer risk in the Chinese population. PMID: 30128886
a genetic variant in the CD3EAP gene is associated with outcome of patients treated for multiple myeloma PMID: 26568154
These findings demonstrate that 19p13.3-GADD45B rs7354 variant and interaction between 19p13.3-GADD45B rs3783501 and 19q13.3-CD3EAP rs967591 may play a role in association with smoke-exposed lung cancer among Chinese. PMID: 28870783
There was no interaction between NFKB1 polymorphisms and PPP1R13L and CD3EAP and smoking status in relation to lung cancer risk PMID: 25917613
In nonsmoking Chinese women CD3EAP rs967591 variant allele carriers are at increased risk of lung cancer. PMID: 23624123
Polymorphism in RAI and CD3EAP are associated with outcome of myeloma patients treated with high dose treatment. PMID: 21046104
model for CAST/hPAF49 function in which the network of interactions of Pol I-specific subunits with UBF facilitates conformational changes of the polymerase, leading to stabilization of the Pol I-template complex and, thereby, activation of transcription PMID: 16809778
The ASE-1 polymorphism was associated with an increased risk of adenomas, OR of 1.39 (95% CI 1.06-1.81), which upon stratification was apparent among women only, OR of 1.66 (95% CI 1.15-2.39) PMID: 16817948
ASE-1 polymorphisms and the previously identified ASE-1 haplotype are not associated with risk of colorectal cancer. PMID: 18289367
A SNP in the 5' noncoding region of exon 1 at position -21 from the first codon, which does not affect the final AA sequence but may affect transcription/translation, was associated with drug sensitivity in the NC160 human cancer cell line panel. PMID: 19396015
ASE-1 co-localises with the RNA polymerase I transcription initiation factor UBF/NOR-90 throughout all stages of the cell cycle and these two proteins associate with each other in vitro. PMID: 9426281

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Subcellular Location

Nucleus, nucleolus. Chromosome. Note=Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes.

Protein Families

Eukaryotic RPA34 RNA polymerase subunit family

Database Links

HGNC: 24219

OMIM: 107325

KEGG: hsa:10849

STRING: 9606.ENSP00000310966

UniGene: Hs.710495

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