CD99L2 Antibody

Datasheet

Code	CSB-PA840569LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: SH-SY5Y whole cell lysate All lanes: CD99L2 antibody at 4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 28, 23, 21, 16, 29 kDa Observed band size: 28 kDa IHC image of CSB-PA840569LA01HU diluted at 1:400 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. Immunofluorescence staining of A549 cells with CSB-PA840569LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CD99L2 Polyclonal antibody

Uniprot No.

Q8TCZ2

Target Names

CD99L2

Alternative Names

C99L2_HUMAN antibody; CD99 antibody; CD99 antigen-like protein 2 antibody; CD99L2 antibody; MIC2-like protein 1 antibody; MIC2L1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human CD99 antigen-like protein 2 protein (26-145AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CD99L2 Antibody (Product code: CSB-PA840569LA01HU) is Non-conjugated. For CD99L2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA840569LB01HU	CD99L2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA840569LC01HU	CD99L2 Antibody, FITC conjugated
Biotin	CSB-PA840569LD01HU	CD99L2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Plays a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Homophilic adhesion molecule, but these interactions may not be required for cell aggregation.

Gene References into Functions

This study identified predicted pathogenic, hemizygous variants on chromosome X in disease genes CD99L2. PMID: 25666757

Subcellular Location

Cell membrane; Single-pass type I membrane protein; Extracellular side. Cell junction.

Protein Families

CD99 family

Tissue Specificity

Expressed in many tissues, with low expression in thymus.

Database Links

HGNC: 18237

OMIM: 300846

KEGG: hsa:83692

STRING: 9606.ENSP00000359403

UniGene: Hs.522805

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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