CD99L2 Antibody

Code CSB-PA840569LA01HU
Size US$166
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Image
  • Western Blot
    Positive WB detected in: SH-SY5Y whole cell lysate
    All lanes: CD99L2 antibody at 4µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 28, 23, 21, 16, 29 kDa
    Observed band size: 28 kDa

  • IHC image of CSB-PA840569LA01HU diluted at 1:400 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system.

  • Immunofluorescence staining of A549 cells with CSB-PA840569LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) CD99L2 Polyclonal antibody
Uniprot No.
Target Names
CD99L2
Alternative Names
C99L2_HUMAN antibody; CD99 antibody; CD99 antigen-like protein 2 antibody; CD99L2 antibody; MIC2-like protein 1 antibody; MIC2L1 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human CD99 antigen-like protein 2 protein (26-145AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The CD99L2 Antibody (Product code: CSB-PA840569LA01HU) is Non-conjugated. For CD99L2 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA840569LB01HU CD99L2 Antibody, HRP conjugated ELISA
FITC CSB-PA840569LC01HU CD99L2 Antibody, FITC conjugated
Biotin CSB-PA840569LD01HU CD99L2 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:200-1:500
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Plays a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Homophilic adhesion molecule, but these interactions may not be required for cell aggregation.
Gene References into Functions
  1. This study identified predicted pathogenic, hemizygous variants on chromosome X in disease genes CD99L2. PMID: 25666757
Subcellular Location
Cell membrane; Single-pass type I membrane protein; Extracellular side. Cell junction.
Protein Families
CD99 family
Tissue Specificity
Expressed in many tissues, with low expression in thymus.
Database Links

HGNC: 18237

OMIM: 300846

KEGG: hsa:83692

STRING: 9606.ENSP00000359403

UniGene: Hs.522805

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