CEP97 Antibody

Datasheet
Code CSB-PA808994LA01HU
See More Details
Size US$299
Uniprot No. Q8IW35
Image
  • Immunofluorescence staining of Hela cells with CSB-PA808994LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Protocols ELISA Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human Centrosomal protein of 97 kDa protein (719-854AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IF; Recommended dilution: IF:1:50-1:200
Relevance Acts as a key negative regulator of ciliogenesis in collaboration with CCP110 by capping the mother centriole thereby preventing cilia formation. Required for recruitment of CCP110 to the centrosome.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Centrosomal protein of 97 kDa (Cep97) (Leucine-rich repeat and IQ domain-containing protein 2), CEP97, LRRIQ2
Immunogen Species Human
Research Area Cell Biology
Target Names CEP97
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Acts as a key negative regulator of ciliogenesis in collaboration with CCP110 by capping the mother centriole thereby preventing cilia formation. Required for recruitment of CCP110 to the centrosome.
Subcellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Database Links

HGNC: 26244

OMIM: 615864

KEGG: hsa:79598

STRING: 9606.ENSP00000342510

UniGene: Hs.444135

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