CFHR5 Antibody

Datasheet
Code CSB-PA883624LA01HU
Size US$299
Uniprot No. Q9BXR6
Image
  • Western Blot
    Positive WB detected in: K562 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate
    All lanes: CFHR5 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 65 kDa
    Observed band size: 65 kDa

  • IHC image of CSB-PA883624LA01HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunogen Recombinant Human Complement factor H-related protein 5 protein (374-569AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200
Relevance Involved in complement regulation. The dimerized forms have avidity for tissue-bound complement fragments and efficiently compete with the physiological complement inhibitor CFH.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias Complement factor H-related protein 5 (FHR-5), CFHR5, CFHL5 FHR5
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Research Area Immunology
Target Names CFHR5
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Involved in complement regulation. The dimerized forms have avidity for tissue-bound complement fragments and efficiently compete with the physiological complement inhibitor CFH.
Involvement in disease CFHR5 deficiency (CFHR5D)
Subcellular Location Secreted
Tissue Specificity Expressed by the liver and secreted in plasma.
Database Links

HGNC: 24668

OMIM: 608593

KEGG: hsa:81494

STRING: 9606.ENSP00000256785

UniGene: Hs.282594

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