CHKB Antibody

Datasheet

Code	CSB-PA005354LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Rat liver tissue, Mouse liver tissue All lanes: CHKB antibody at 6.6μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 46, 14 kDa Observed band size: 46 kDa IHC image of CSB-PA005354LA01HU diluted at 1:300 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA005354LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CHKB Polyclonal antibody

Uniprot No.

Q9Y259

Target Names

CHKB

Alternative Names

CHETK antibody; Chetk-beta antibody; Chkb antibody; Chkb choline kinase beta antibody; Chkb protein antibody; CHKB_HUMAN antibody; CHKL antibody; Choline kinase beta antibody; Choline kinase-like antibody; Choline kinase-like protein antibody; Choline/ethanolamine kinase antibody; choline/ethanolamine kinase beta antibody; Choline/ethanolamine kinase; beta antibody; CK antibody; CK/EK antibody; CK/EK-beta antibody; CKB antibody; CKEKB antibody; EK antibody; EKB antibody; Ethanolamine kinase antibody; Ethanolamine kinase beta antibody; Ethanolamine kinase; beta antibody; MDCMC antibody

Raised in

Rabbit

Species Reactivity

Human, Rat, Mouse

Immunogen

Recombinant Human Choline/ethanolamine kinase protein (2-99AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CHKB Antibody (Product code: CSB-PA005354LA01HU) is Non-conjugated. For CHKB Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA005354LB01HU	CHKB Antibody, HRP conjugated	ELISA
FITC	CSB-PA005354LC01HU	CHKB Antibody, FITC conjugated
Biotin	CSB-PA005354LD01HU	CHKB Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:100-1:300
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Has a key role in phospholipid metabolism, and catalyzes the first step of phosphatidylethanolamine and phosphatidylcholine biosynthesis.

Gene References into Functions

findings suggest that CKbeta, in concert with CKalpha, and depending on its phosphorylation status, might play a critical role as a druggable target in carcinogenesis PMID: 27149373
The whole exome sequencing revealed a novel homozygous missense mutation in the Choline Kinase-Beta (CHKB) gene in exon 9. PMID: 27123443
A new form of congenital muscular dystrophy with multisystem involvement and characteristic mitochondrial structural changes, due to choline kinase beta gene defects has been characterized. PMID: 26067811
A novel silent variant in the choline kinase beta causing muscular dystrophy. PMID: 25740612
Its mutations cause congenital muscular dystrophy.[Review] PMID: 24291895
study identified a novel haplotype consisting of the indel variation, which had not been detected in previous studies in Japanese and Korean populations, and observed four single-nucleotide polymorphisms in CHKB/CPT1B PMID: 24571861
CHKB encodes choline kinase beta, an enzyme that catalyzes the first de-novo biosynthetic step of phosphatidylcholine, the most abundant phospholipid in the eukaryotic membrane [review] PMID: 23945283
CHKB activity was reduced in all three patients, significant reduction in choline:N-acetyl aspartate and choline:creatine ratios in keeping with a general decrease in the amount of choline and phosphocholine-based substrate PMID: 23692895
The study extends on the observation of a strong multiethnic association of polymorphisms in the TCRA and P2RY11 with narcolepsy, but does not confirm the association of CPT1B/CHKB (rs5770917) in the Chinese population. PMID: 22177342
homozygous or compound heterozygous mutations in the gene encoding choline kinase beta were identified in individulas with congenital muscular dystrophy. PMID: 21665002
A single nucleotide polymorphism located between CPT1B and CHKB, was associated with narcolepsy in Japanese (rs5770917[C], odds ratio (OR) = 1.79, combined P = 4.4 x 10(-7)) and other ancestry groups (OR = 1.40, P = 0.02). PMID: 18820697
Japanese CNS hypersomnias (essential hypersomnia: EHS) other than narcolepsy with cataplexy was significantly associated with SNP rs5770917 (located between CPT1B and CHKB) and HLA-DRB1*1501-DQB1*0602 haplotype PMID: 19404393

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Protein Families

Choline/ethanolamine kinase family

Database Links

HGNC: 1938

OMIM: 612395

KEGG: hsa:1120

STRING: 9606.ENSP00000384400

UniGene: Hs.654827

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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