CHODL Antibody

Datasheet

Code	CSB-PA005370LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate All lanes: CHODL antibody at 6.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 31, 26, 28, 29 kDa Observed band size: 31 kDa IHC image of CSB-PA005370LA01HU diluted at 1:300 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA005370LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CHODL Polyclonal antibody

Uniprot No.

Q9H9P2

Target Names

CHODL

Alternative Names

C21orf68 antibody; Chodl antibody; CHODL_HUMAN antibody; Chondrolectin antibody; Chromosome 21 open reading frame 68 antibody; FLJ12627 antibody; MT75 antibody; PRED12 antibody; Transmembrane protein MT75 antibody; UNQ872/PRO1890 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Chondrolectin protein (105-206AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CHODL Antibody (Product code: CSB-PA005370LA01HU) is Non-conjugated. For CHODL Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA005370LB01HU	CHODL Antibody, HRP conjugated	ELISA
FITC	CSB-PA005370LC01HU	CHODL Antibody, FITC conjugated
Biotin	CSB-PA005370LD01HU	CHODL Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

May play a role in the development of the nervous system such as in neurite outgrowth and elongation. May be involved in motor axon growth and guidance.

Gene References into Functions

Data indicate that chondrolectin (CHODL) is likely to be a prognostic biomarker in the clinic and targeting CHODL might be a strategy for the development of anticancer drugs. PMID: 22016508
cloning and characterization; localized at chromosome 21q21 PMID: 12079284
study describes the molecular characterization of novel members of the chondrolectin family associated with T cell maturation and a subcellular localization of chondrolectin f isoform in the endoplasmic reticulum-Golgi apparatus PMID: 12621022
CHODLDeltaE/CHODLfDeltaE protein variant localizes in the late endoplasmic reticulum and is detected in spleen and tonsils; the isoform seems to be differentially expressed in thymocytes and lymphocytes. PMID: 17606388

Subcellular Location

Cytoplasm. Membrane; Single-pass type I membrane protein.; [Isoform 2]: Endoplasmic reticulum. Endoplasmic reticulum membrane; Single-pass type I membrane protein.; [Isoform 3]: Cytoplasm.

Tissue Specificity

Found in spleen, testis, prostate and fetal liver. Expression limited to vascular muscle of testis, smooth muscle of prostate stroma, heart muscle, skeletal muscle, crypts of small intestine, and red pulp of spleen. B lymphocytes express isoform 2 only; p

Database Links

HGNC: 17807

OMIM: 607247

KEGG: hsa:140578

STRING: 9606.ENSP00000299295

UniGene: Hs.283725

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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