CHP1 Antibody

Datasheet

Code	CSB-PA860773LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Rat kidney tissue, Mouse brain tissue All lanes: CHP1 antibody at 2.4μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 23 kDa Observed band size: 23 kDa IHC image of CSB-PA860773LA01HU diluted at 1:600 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescent analysis of Hela cells using CSB-PA860773LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CHP1 Polyclonal antibody

Uniprot No.

Q99653

Target Names

CHP1

Alternative Names

Calcineurin B homolog antibody; Calcineurin B homologous protein antibody; Calcineurin homologous protein antibody; Calcineurin like EF hand protein 1 antibody; Calcium binding protein CHP antibody; Calcium binding protein p22 antibody; Calcium-binding protein CHP antibody; Calcium-binding protein p22 antibody; chp antibody; CHP1_HUMAN antibody; Sid 470 antibody; Sid470 antibody; SLC9A1 binding protein antibody; SLC9A1BP antibody; Wrch2 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human Calcineurin B homologous protein 1 protein (2-195AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CHP1 Antibody (Product code: CSB-PA860773LA01HU) is Non-conjugated. For CHP1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA860773LB01HU	CHP1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA860773LC01HU	CHP1 Antibody, FITC conjugated
Biotin	CSB-PA860773LD01HU	CHP1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:50-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Calcium-binding protein involved in different processes such as regulation of vesicular trafficking, plasma membrane Na(+)/H(+) exchanger and gene transcription. Involved in the constitutive exocytic membrane traffic. Mediates the association between microtubules and membrane-bound organelles of the endoplasmic reticulum and Golgi apparatus and is also required for the targeting and fusion of transcytotic vesicles (TCV) with the plasma membrane. Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Affects the pH sensitivity of SLC9A1/NHE1 by increasing its sensitivity at acidic pH. Required for the stabilization and localization of SLC9A1/NHE1 at the plasma membrane. Inhibits serum- and GTPase-stimulated Na(+)/H(+) exchange. Plays a role as an inhibitor of ribosomal RNA transcription by repressing the nucleolar UBF1 transcriptional activity. May sequester UBF1 in the nucleoplasm and limit its translocation to the nucleolus. Associates to the ribosomal gene promoter. Acts as a negative regulator of the calcineurin/NFAT signaling pathway. Inhibits NFAT nuclear translocation and transcriptional activity by suppressing the calcium-dependent calcineurin phosphatase activity. Also negatively regulates the kinase activity of the apoptosis-induced kinase STK17B. Inhibits both STK17B auto- and substrate-phosphorylations in a calcium-dependent manner.

Gene References into Functions

serum-independent activation of NHE1 by bound CHP2 is one of the key mechanisms for the maintenance of high pH(i) and the resistance to serum deprivation-induced cell death in malignantly transformed cells PMID: 12226101
CHP is important for pH(i)-dependent regulation of Na+/H+ exchanger 1 (NHE1) via tightly bound Ca2+ ions which serve as a crucial structural elements required for this role. PMID: 15035633
crystallographic analysis of the human calcineurin homologous protein CHP2 bound to the cytoplasmic region of the Na+/H+ exchanger NHE1 PMID: 16511206
helix formation of the cytoplasmic region of NHE1 by calcineurin B homologous protein 1 is a prerequisite for generating the active form of NHE1 PMID: 17050540
CHP2 has a role in tumorigenesis and as an activator of the calcineurin/NFAT signaling pathway PMID: 18815128

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Subcellular Location

Nucleus. Cytoplasm. Cytoplasm, cytoskeleton. Endomembrane system. Endoplasmic reticulum-Golgi intermediate compartment. Endoplasmic reticulum. Cell membrane. Membrane; Lipid-anchor.

Protein Families

Calcineurin regulatory subunit family, CHP subfamily

Tissue Specificity

Ubiquitously expressed. Has been found in fetal eye, lung, liver, muscle, heart, kidney, thymus and spleen.

Database Links

HGNC: 17433

OMIM: 606988

KEGG: hsa:11261

STRING: 9606.ENSP00000335632

UniGene: Hs.406234

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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