CSNK1G1 Antibody

Datasheet
Code CSB-PA888030EA01HU
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Size US$299
Uniprot No. Q9HCP0
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate
    All lanes: CSNK1G1 antibody at 3.4µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 49, 46 kDa
    Observed band size: 49 kDa

  • IHC image of CSB-PA888030EA01HU diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunogen Recombinant Human Casein kinase I isoform gamma-1 protein (1-125AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500
Relevance Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Regulates fast synaptic transmission mediated by glutamate (By similarity). Phosphorylates CLSPN.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Casein kinase I isoform gamma-1 (CKI-gamma 1) (EC 2.7.11.1), CSNK1G1
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Research Area Signal Transduction
Target Names CSNK1G1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Regulates fast synaptic transmission mediated by glutamate (By similarity). Phosphorylates CLSPN.
Subcellular Location Cytoplasm
Protein Families Protein kinase superfamily, CK1 Ser/Thr protein kinase family, Casein kinase I subfamily
Database Links

HGNC: 2454

OMIM: 606274

KEGG: hsa:53944

STRING: 9606.ENSP00000305777

UniGene: Hs.646508

Pathway Hedgehog signaling pathway

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