Product Details

Uniprot No.

Target Names

CUL4A

Alternative Names

2810470J21Rik antibody; AW495282 antibody; CUL 4A antibody; CUL-4A antibody; Cul4a antibody; Cul4a protein antibody; CUL4A_HUMAN antibody; Cullin-4A antibody; MGC36573 antibody; MGC64071 antibody

Species Reactivity

Human,Mouse,Rat

Immunogen

Human CUL4A

Immunogen Species

Homo sapiens (Human)

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.

Tested Applications

ELISA,IHC

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of p53/TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(DTL) directs autoubiquitination of DTL. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1. A number of DCX complexes (containing either TRPC4AP or DCAF12 as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. The DCX(AMBRA1) complex is a master regulator of the transition from G1 to S cell phase by mediating ubiquitination of phosphorylated cyclin-D (CCND1, CCND2 and CCND3). The DCX(AMBRA1) complex also acts as a regulator of Cul5-RING (CRL5) E3 ubiquitin-protein ligase complexes by mediating ubiquitination and degradation of Elongin-C (ELOC) component of CRL5 complexes. With CUL4B, contributes to ribosome biogenesis.

Gene References into Functions

The replication initiation determinant protein PHIP modulates replication by recruiting CUL4 to chromatin. PMID: 30018425
Downregulation of CUL4A expression inhibited cell proliferation, migration and invasion, and increased the percentage of cell apoptosis, in HEPG2 and MHCC97H cells, while CUL4A overexpression led to the opposite effects. PMID: 30015884
Results suggested that miR377 is a significant negative regulator of CUL4A that controls cancer cell progression in ovarian cancer cell lines. PMID: 29512715
These results suggest that different DDB1-CUL4 associated factors play distinct roles in human lung adenocarcinoma development. PMID: 28336923
The identification of CUL4A as a downstream target of TGF-beta1 represents a critical pro-survival mechanism in breast cancer progression and provides another point for therapeutic intervention in breast cancer. PMID: 28902348
Our data demonstrate that overexpression of CUL4A plays an oncogenic role in iCCA and adversely affects disease-free survival. Thus, it may prove to be a powerful prognostic factor and a potential therapeutic target. PMID: 28576144
JMJD2C regulated the activities of lung cancer cells by directly controlling the expression of CUL4A in JMJD2C over-expression cell line. PMID: 28236704
plays a critical role in perihilar cholangiocarcinoma (PHCC) metastasis by facilitating PHCC cell motility and cell invasion as well as consequent induction of epithelial to mesenchymal transition via, at least partially, up-regulating transcriptional regulation factor ZEB1 PMID: 28428711
The CUL4A interacts with WD-40 proteins through the adaptor protein DNA damage-binding protein 1 (DDB1) to target substrates for ubiquitylation. PMID: 28886238
knockdown of cullin 4A via small interfering RNA inhibited the proliferation of the multiple myeloma cell lines by delaying cell-cycle progression and increasing apoptosis. cullin 4A downregulation inhibited multiple myeloma cell migration and invasion in vitro. Our results suggested that cullin 4A could be a promising therapy target in multiple myeloma patients PMID: 28677427
decreased tumour growth and increased chemosensitivity to gemcitabine were also noted after Cul4A knockdown. PMID: 26969027
CUL4A played an oncogene role through ZEB1 in IL-6-induced colorectal carcinoma progression. PMID: 27418574
Overexpression of miR-494 inhibited proliferation, migration, invasion and EMT of ovarian cancer cells by directly suppressing CUL4A expression. Therefore, our findings indicate that miR-494/CUL4A axis is important in the control of ovarian cancer tumorigenesis. PMID: 27983981
CUL4A sensitizes colorectal cancer cells to cisplatin induced cell death. PMID: 28095394
Elevated expression of CUL4A is positively correlated with distant metastases. PMID: 26055549
Increased CUL4A expression is associated with osteosarcoma. PMID: 26715273
Findings indicate the importance of a microRNAs miR-9/137-CUL4A-Hippo signaling axis in gastric cancer (GC), and suggest new therapeutic targets for future treatment of GC. PMID: 26840256
CUL4A facilitates hepatocarcinogenesis by promoting cell cycle progression and epithelial-mesenchymal transition. PMID: 26593394
this study proposes that inflammation-induced Jak-STAT3 signaling leads to colon cancer development through proteasomal degradation of DICER1 by ubiquitin ligase complex of CUL4A(DCAF1), which suggests a novel therapeutic opportunity for colon cancer PMID: 26965998
We observed that knockdown of Cul4A was associated with increased sensitivity to gemcitabine through upregulation of TGFBI in lung cancer cells. PMID: 26503734
Amplification of CUL4A, IRS2, and TFDP1 genes showed a significant difference in disease-free survival by both univariate and multivariate survival analyses in intrahepatic cholangiocarcinoma. PMID: 26684807
These results suggest a linkage between Cul4A and Gli1 expression in human mesothelioma. PMID: 26218750
PR-Set7 is a direct target of Cul4A for degradation and involved in the formation of lung tumors in the conditional Cul4A transgenic mouse model. PMID: 26104890
CUL4A regulated EGFR transcriptional expression. PMID: 25413624
eEF1A1 may mediate SAMHD1 turnover by targeting it to the proteosome for degradation through association with Cullin4A and Rbx1. PMID: 25423367
Our results suggest a synergistic effect between CUL4A high levels and the activation of the RAS pathway in the tumorigenesis of basal-like breast cancer tumors PMID: 24870930
we observed an increased level of polyubiquitination on p53 in CUL4B overexpressing stable cell line upon treatment with siRNA specific for CUL4A indicating that CUL4B plays a vital role in p53 stability. PMID: 24452595
dysfunctional Pex7p, including mutants from RCDP patients, is degraded by a ubiquitin-dependent proteasomal pathway involving the CRL4A (Cullin4A-RING ubiquitin ligase) complex. PMID: 24989250
The results of this study indicated that CUL4A enhances pituitary cell proliferation, migration and invasion and may thus contribute to pituitary tumor development and progression. PMID: 24420924
CUL4A ubiquitin ligase plays a key role in a wide range of cellular processes, including the cell cycle, chromatin remodelling, DNA damage response, DNA replication, spermatogenesis and haematopoiesis. PMID: 24522884
CUL4A and ERK1/2 participated in multi-drug resistance in breast cancer through regulation of MDR1/P-gp expression. PMID: 24368600
Findings indicate that Cul4A is oncogenic in vivo and that Cul4A over-expression is associated with cisplatin resistance in lung cancer cells. PMID: 24648314
CUL4A has a pivotal role in regulating the metastatic behavior of breast cancer cells in a process involving ZEB1 PMID: 24305877
These results indicate that both yeast Rtt101Mms1 and human Cul4ADDB1 are required for efficient deposition of new H3 onto DNA. PMID: 24209620
DLC1 was ubiquitinated and degraded by cullin 4A-RING ubiquitin ligase (CRL4A) complex interaction with DDB1 and the FBXW5 substrate receptor. PMID: 24082123
p73 interacts with the CDL4A complex by binding directly to DDB1. The CDL4A complex is able to monoubiquitylate p73, negatively affecting its transcriptional function. PMID: 23085759
The EZH2-DCAF1/DDB1/CUL4 represents a previously unrecognized methylation-dependent ubiquitination machinery specifically recognizing "methyl degron"; nonhistone protein stability can be dynamically regulated in a methylation-dependent manner. PMID: 23063525
potential GRK5 interacting proteins and the association of GRK5 with DDB1 in cell and the regulation of GRK5 level by DDB1-CUL4 ubiquitin ligase complex-dependent proteolysis pathway PMID: 22952844
subjects with tumors that highly expressed CUL4A had poor overall survival and CUL4A downregulation inhibited cell proliferation and induced apoptosis in vitro and in vivo. PMID: 22422151
Findings indicate structural and conformational insights of the DDB1-CUL4A(DDB2) E3 ligase, with significant implications for the regulation and overall organization of the proteins responsible for initiation of nucleotide-excision repair (NER) pathway. PMID: 22822215
Activation of EGFR inhibits the interaction of PCNA with CUL4A, whereas inhibition of EGFR leads to increased CUL4A-PCNA interaction and CUL4A-dependent ubiquitin-mediated degradation of PCNA PMID: 22692198
Artemis and DDB2 regulate p27 via the Cul4A-based E3 ligase complex. PMID: 22134138
study indicates that Cul4A amplification and overexpression play an oncogenic role in the pathogenesis of mesothelioma PMID: 19929949
hCUL4A suppresses apoptosis and DNA damage by regulating apoptosis-related proteins and cell cycle regulators (Bcl-2, caspase-3, p53 and p27); consequently, hCUL4A promotes cell survival. PMID: 22120631
Studies indicate the modular architecture of DDB1-CUL4 in complex with DDB2, CSA and CDT2 in DNA repair of UV-induced DNA lesions. PMID: 21550341
the CUL4A.DDB1 E3 complex is important for regulation of RASSF1A during mitosis, and it may contribute to inactivation of RASSF1A and promoting cell cycle progression PMID: 21205828
13q34 amplification may be of relevance in tumor progression of breast cancers by inducing overexpression of CUL4A and TFDP1, important in cell cycle regulation. These genes were also overexpressed in non-basal-like tumor samples. PMID: 19995430
DDB1 modulates the function of APC/C(Cdh1) in a manner independent of the Cul4-DDB1 complex PMID: 20395298
TFDP1, CUL4A, and CDC16 are probable targets of an amplification mechanism and therefore may be involved, together or separately, in development and/or progression of some hepatocellular carcinomas PMID: 12029633
DET1 promotes ubiquitination and degradation of c-Jun by assembling a multisubunit ubiquitin ligase containing DNA Damage Binding Protein-1 (DDB1), cullin 4A (CUL4A), Regulator of Cullins-1 (ROC1), and constitutively photomorphogenic-1 PMID: 14739464