Crotonyl-HIST1H2BC (K12) Antibody

Datasheet
Code CSB-PA010403OA12crHU
Size US$319
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, 293 whole cell lysate (all treated with 30mM sodium crotonylate for 4h)
    All lanes: HIST1H2BC antibody at 0.97µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 14 kDa
    Observed band size: 14 kDa

  • Immunofluorescence staining of Hela cells (treated with 30mM sodium crotonylate for 4h) with CSB-PA010403OA12crHU at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunoprecipitating HIST1H2BC in 293 whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-PA010403OA12crHU in 293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-PA010403OA12crHU (5µg) + 293 whole cell lysate (500µg)
    Lane 3: 293 whole cell lysate (20µg)

  • Chromatin Immunoprecipitation Hela (106, treated with 30mM sodium crotonylate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2BC (CSB-PA010403OA12crHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

Product Details

Full Product Name Rabbit anti-Human HIST1H2BC Polyclonal antibody
Uniprot No. P62807
Target Names HIST1H2BC
Alternative Names Histone H2B type 1-C/E/F/G/I (Histone H2B.1 A) (Histone H2B.a) (H2B/a) (Histone H2B.g) (H2B/g) (Histone H2B.h) (H2B/h) (Histone H2B.k) (H2B/k) (Histone H2B.l) (H2B/l), HIST1H2BC; HIST1H2BE; HIST1H2BF; HIST1H2BG; HIST1H2BI, H2BFL; H2BFH; H2BFG; H2BFA; H2BFK
Raised in Rabbit
Species Reactivity Human
Immunogen Peptide sequence around site of Crotonyl-Lys (12) derived from Human Histone H2B type 1-C/E/F/G/I
Immunogen Species Human
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Antigen Affinity Purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IF, IP, ChIP
Recommended Dilution
Application Recommended Dilution
WB 1:100-1:1000
IF 1:1-1:10
IP 1:200-1:2000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Target Data

Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.; FUNCTION
Subcellular Location Nucleus, Chromosome
Protein Families Histone H2B family
Database Links

HGNC: 4757

OMIM: 602798

KEGG: hsa:3017

STRING: 9606.ENSP00000366962

UniGene: Hs.182137

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