D8L Antibody

Code CSB-PA322653LA01VAA
Size US$166
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  • Western Blot
    Positive WB detected in Recombinant protein
    All lanes: D8L antibody at 3µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 52 kDa
    Observed band size: 52 kDa

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Product Details

Full Product Name
Rabbit anti-Vaccinia virus D8L Polyclonal antibody
Uniprot No.
Target Names
D8L
Alternative Names
D8LCell surface-binding protein antibody; Carbonic anhydrase homolog antibody
Raised in
Rabbit
Species Reactivity
Vaccinia virus
Immunogen
Recombinant Vaccinia virus Cell surface-binding protein (1-304AA)
Immunogen Species
Vaccinia virus
Conjugate
Non-conjugated

The D8L Antibody (Product code: CSB-PA322653LA01VAA) is Non-conjugated. For D8L Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA322653LB01VAA D8L Antibody, HRP conjugated ELISA
FITC CSB-PA322653LC01VAA D8L Antibody, FITC conjugated
Biotin CSB-PA322653LD01VAA D8L Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The D8L polyclonal antibody is produced by injecting rabbits with the recombinant Vaccinia virus Cell surface-binding protein (1-304aa), which serves as the immunogen. The rabbits then produce antibodies against the Vaccinia virus D8L protein. These antibodies are purified from the rabbit serum using protein G purification. The purity is 95%+. The resulting purified rabbit anti-Vaccinia virus D8L polyclonal antibody is highly specific to Vaccinia virus D8L and has a high affinity for the antigen. This D8L antibody is suitable for use in ELISA and WB.

The Vaccinia virus cell surface-binding protein D8L mainly protects the virus from the host's immune system by inhibiting complement-mediated lysis and modulating the host's inflammatory response. D8L can reduce the host's inflammatory response and help the virus evade immune detection by binding to and inactivating complement component C3b and inhibiting the activity of chemokines involved in the recruitment of immune cells.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 1 reviews

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Applications : Western blot analysis(WB)

Sample type: cell

Review: Western blot analysis of lysates prepared with HeLa cells 24 h after infection with MVA or MVA-NS3 (MOI 5) using antibodies against VACV D8.

By Anonymous

Target Background

Function
Binds to chondroitin sulfate on the cell surface to provide virion attachment to target cell.
Subcellular Location
Virion membrane.
Protein Families
Alpha-carbonic anhydrase family
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