DAD1 Antibody

Code CSB-PA03124A0Rb
Size US$166
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  • Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA03124A0Rb at dilution of 1:100
  • Immunofluorescent analysis of HepG2 cells using CSB-PA03124A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) DAD1 Polyclonal antibody
Uniprot No.
Target Names
DAD1
Alternative Names
DAD1; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; Oligosaccharyl transferase subunit DAD1; Defender against cell death 1; DAD-1
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 protein (1-113AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The DAD1 Antibody (Product code: CSB-PA03124A0Rb) is Non-conjugated. For DAD1 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA03124B0Rb DAD1 Antibody, HRP conjugated ELISA
FITC CSB-PA03124C0Rb DAD1 Antibody, FITC conjugated
Biotin CSB-PA03124D0Rb DAD1 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. Required for the assembly of both SST3A- and SS3B-containing OST complexes. Loss of the DAD1 protein triggers apoptosis.
Gene References into Functions
  1. this study shows that abnormal expression of DAD1 may relate to the hyperfunction of immune responses and excessive apoptosis of severe aplastic anemia CD34(+) cells PMID: 27086042
  2. single-nucleotide polymorphisms in DAD1 gene is associated with neuroendocrine tumor. PMID: 21606320
  3. DAD1 gene expression is decreased in follicular variant of papillary thyroid carcinoma. PMID: 21509594
  4. DAD1 protein overexpression is associated with small bowel carcinoid tumors PMID: 18383209
Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
DAD/OST2 family
Database Links

HGNC: 2664

OMIM: 600243

KEGG: hsa:1603

STRING: 9606.ENSP00000250498

UniGene: Hs.82890

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