DAZAP2 Antibody

Datasheet
Code CSB-PA622986LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. Q15038
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, A375 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, SH-SY5Y whole cell lysate, Rat brain tissue
    All lanes: DAZAP2 antibody at 3.5µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 18, 17, 16, 14, 10, 23 kDa
    Observed band size: 18 kDa

  • IHC image of CSB-PA622986LA01HU diluted at 1:400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of MCF-7 cells with CSB-PA622986LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human DAZ-associated protein 2 protein (1-168AA)
Raised in Rabbit
Species Reactivity Human, Rat
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200
Relevance nuclear speck, identical protein binding, WW domain binding
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias DAZ-associated protein 2 (Deleted in azoospermia-associated protein 2), DAZAP2, KIAA0058
Immunogen Species Human
Research Area Epigenetics and Nuclear Signaling
Target Names DAZAP2
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Subcellular Location Cytoplasm, Nucleus
Tissue Specificity Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Down-regulated in multiple myeloma.
Database Links

HGNC: 2684

OMIM: 607431

KEGG: hsa:9802

STRING: 9606.ENSP00000448051

UniGene: Hs.369761

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