DBF4B Antibody

Datasheet

Code	CSB-PA822784LA01HU
Size	US$166
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Image	Western blot All lanes: DBF4B antibody at 4µg/ml Lane 1: MCF-7 whole cell lysate Lane 2: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 68, 48, 55, 19 kDa Observed band size: 48 kDa Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA822784LA01HU at dilution of 1:100
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) DBF4B Polyclonal antibody

Uniprot No.

Q8NFT6

Target Names

DBF4B

Alternative Names

Activator of S phase kinase like protein 1 antibody; Activator of S phase kinase-like protein 1 antibody; ASK like protein 1 antibody; ASK-like protein 1 antibody; ASKL1 antibody; chifb antibody; Chiffon homolog B antibody; DBF 4B antibody; DBF4 homolog B (S. cerevisiae) antibody; DBF4 homolog B antibody; Dbf4 related factor 1 antibody; Dbf4-related factor 1 antibody; dbf4b antibody; DBF4B_HUMAN antibody; DRF1 antibody; FLJ13087 antibody; MGC15009 antibody; Protein DBF4 homolog B antibody; ZDBF1B antibody; Zinc finger DBF type containing 1B antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Protein DBF4 homolog B protein (1-300AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The DBF4B Antibody (Product code: CSB-PA822784LA01HU) is Non-conjugated. For DBF4B Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA822784LB01HU	DBF4B Antibody, HRP conjugated	ELISA
FITC	CSB-PA822784LC01HU	DBF4B Antibody, FITC conjugated
Biotin	CSB-PA822784LD01HU	DBF4B Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle.

Gene References into Functions

Knockdown of the exon6-containing isoform (DBF4B-FL) significantly inhibits the tumorigenic potential of colon cancer. Overexpression of DBF4B-FL protects against DNA damage induced by SRSF1 knockdown. PMID: 29262322
Results describe a novel human protein, Drf1, related to both human and yeast Dbf4. Drf1 is a nuclear cell cycle-regulated protein which binds to Cdc7 and activates the kinase. PMID: 12065429
ASKL1 in a complex with Cdc7 may play a role in normal progression of both S and M phases PMID: 15668232
These results indicate that Ddk functions as an upstream regulator to monitor S-phase checkpoint signaling. PMID: 19111665

Subcellular Location

Nucleus. Note=Predominantly found in soluble fraction but not in the chromatin-bound fraction.

Tissue Specificity

Widely expressed. Highly expressed in testis.

Database Links

HGNC: 17883

OMIM: 611661

KEGG: hsa:80174

STRING: 9606.ENSP00000323663

UniGene: Hs.369998

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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