DBF4B Antibody

Code CSB-PA822784LA01HU
Size US$166
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  • Western blot
    All lanes: DBF4B antibody at 4µg/ml
    Lane 1: MCF-7 whole cell lysate
    Lane 2: A549 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 68, 48, 55, 19 kDa
    Observed band size: 48 kDa

  • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA822784LA01HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) DBF4B Polyclonal antibody
Uniprot No.
Target Names
DBF4B
Alternative Names
Activator of S phase kinase like protein 1 antibody; Activator of S phase kinase-like protein 1 antibody; ASK like protein 1 antibody; ASK-like protein 1 antibody; ASKL1 antibody; chifb antibody; Chiffon homolog B antibody; DBF 4B antibody; DBF4 homolog B (S. cerevisiae) antibody; DBF4 homolog B antibody; Dbf4 related factor 1 antibody; Dbf4-related factor 1 antibody; dbf4b antibody; DBF4B_HUMAN antibody; DRF1 antibody; FLJ13087 antibody; MGC15009 antibody; Protein DBF4 homolog B antibody; ZDBF1B antibody; Zinc finger DBF type containing 1B antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Protein DBF4 homolog B protein (1-300AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The DBF4B Antibody (Product code: CSB-PA822784LA01HU) is Non-conjugated. For DBF4B Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA822784LB01HU DBF4B Antibody, HRP conjugated ELISA
FITC CSB-PA822784LC01HU DBF4B Antibody, FITC conjugated
Biotin CSB-PA822784LD01HU DBF4B Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle.
Gene References into Functions
  1. Knockdown of the exon6-containing isoform (DBF4B-FL) significantly inhibits the tumorigenic potential of colon cancer. Overexpression of DBF4B-FL protects against DNA damage induced by SRSF1 knockdown. PMID: 29262322
  2. Results describe a novel human protein, Drf1, related to both human and yeast Dbf4. Drf1 is a nuclear cell cycle-regulated protein which binds to Cdc7 and activates the kinase. PMID: 12065429
  3. ASKL1 in a complex with Cdc7 may play a role in normal progression of both S and M phases PMID: 15668232
  4. These results indicate that Ddk functions as an upstream regulator to monitor S-phase checkpoint signaling. PMID: 19111665
Subcellular Location
Nucleus. Note=Predominantly found in soluble fraction but not in the chromatin-bound fraction.
Tissue Specificity
Widely expressed. Highly expressed in testis.
Database Links

HGNC: 17883

OMIM: 611661

KEGG: hsa:80174

STRING: 9606.ENSP00000323663

UniGene: Hs.369998

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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