DDI2 Antibody

Code CSB-PA732937LA01HU
Size US$166
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  • Western blot
    All lanes: DDI2 antibody at 4µg/ml
    Lane 1: A431 whole cell lysate
    Lane 2: HL60 whole cell lysate
    Lane 3: K562 whole cell lysate
    Lane 4: Caco-2 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 45, 24, 47 kDa
    Observed band size: 47 kDa

  • Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA732937LA01HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) DDI2 Polyclonal antibody
Uniprot No.
Target Names
DDI2
Alternative Names
DDI1 DNA damage inducible 1 homolog 2 antibody; Ddi2 antibody; DDI2_HUMAN antibody; DNA damage inducible 1 homolog 2 (S. cerevisiae) antibody; DNA damage inducible 1 homolog 2 antibody; DNA damage inducible protein 2 antibody; MGC14844 antibody; Protein DDI1 homolog 2 antibody; RP4-680D5.5 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Protein DDI1 homolog 2 protein (1-211AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The DDI2 Antibody (Product code: CSB-PA732937LA01HU) is Non-conjugated. For DDI2 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA732937LB01HU DDI2 Antibody, HRP conjugated ELISA
FITC CSB-PA732937LC01HU DDI2 Antibody, FITC conjugated
Biotin CSB-PA732937LD01HU DDI2 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function
Aspartic protease that mediates the cleavage of NFE2L1/NRF1 at 'Leu-104', thereby promoting release of NFE2L1/NRF1 from the endoplasmic reticulum membrane. Ubiquitination of NFE2L1/NRF1 is a prerequisite for cleavage, suggesting that DDI2 specifically recognizes and binds ubiquitinated NFE2L1/NRF1. Seems to act as a proteasomal shuttle which links the proteasome and replication fork proteins like RTF2 (Probable). Required, with DDI1, for cellular survival following replication stress. Together or redudantly with DDI1, removes RTF2 from stalled forks to allow cell cycle progression after replication stress and maintains genome integrity.
Gene References into Functions
  1. the 3D structures of the human Ddi2 UBL and RVP domains were solved and identified a new helical domain that extends on either side of the Rretroviral protease-like dimer. PMID: 27461074
  2. Here the authors show that the aspartyl protease DNA-damage inducible 1 homolog 2 (DDI2) is required to cleave and activate Nrf1. PMID: 27528193
Subcellular Location
Cytoplasm, cytosol. Chromosome.
Protein Families
DDI1 family
Database Links

HGNC: 24578

KEGG: hsa:84301

STRING: 9606.ENSP00000417748

UniGene: Hs.718857

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