DIRAS1 Antibody

Datasheet

Code	CSB-PA006905LA01HU
Size	US$166
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Image	IHC image of CSB-PA006905LA01HU diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CSB-PA006905LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) DIRAS1 Polyclonal antibody

Uniprot No.

O95057

Target Names

DIRAS1

Alternative Names

DIRAS1 antibody; GBTS1 antibody; RIGGTP-binding protein Di-Ras1 antibody; Distinct subgroup of the Ras family member 1 antibody; Ras-related inhibitor of cell growth antibody; Rig antibody; Small GTP-binding tumor suppressor 1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human GTP-binding protein Di-Ras1 protein (1-195AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The DIRAS1 Antibody (Product code: CSB-PA006905LA01HU) is Non-conjugated. For DIRAS1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA006905LB01HU	DIRAS1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA006905LC01HU	DIRAS1 Antibody, FITC conjugated
Biotin	CSB-PA006905LD01HU	DIRAS1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Displays low GTPase activity and exists predominantly in the GTP-bound form.

Gene References into Functions

This neuronal DIRAS1 gene with a proposed role in cholinergic transmission provides not only a candidate for human myoclonic epilepsy but also insights into the disease etiology, while establishing a spontaneous model for future intervention studies and functional characterization. PMID: 28223533
Results show that DIRAS1 is frequently methylated in human colorectal cancer and the expression of DIRAS1 is silenced by promoter region methylation. PMID: 28491151
DiRas1 expression inhibits malignant features of cancers in part by nonproductively binding to SmgGDS and inhibiting the binding of other small GTPases to SmgGDS PMID: 26814130
DIRAS1 downregulation is associated with esophageal squamous cell carcinoma metastasis. PMID: 23436800
a novel Ras-related protein and potential neural tumor suppressor (RIG) PMID: 12107278

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Subcellular Location

Cell membrane; Lipid-anchor; Cytoplasmic side.

Protein Families

Small GTPase superfamily, Di-Ras family

Tissue Specificity

Highly expressed in heart and brain.

Database Links

HGNC: 19127

OMIM: 607862

KEGG: hsa:148252

STRING: 9606.ENSP00000325836

UniGene: Hs.172753

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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