DMAP1 Antibody

Datasheet

Code	CSB-PA873611LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: MCF-7 whole cell lysate, Colo320 whole cell lysate All lanes: DMAP1 antibody at 11µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53 kDa Observed band size: 53 kDa IHC image of CSB-PA873611LA01HU diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA873611LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) DMAP1 Polyclonal antibody

Uniprot No.

Q9NPF5

Target Names

DMAP1

Alternative Names

DMAP1 antibody; KIAA1425DNA methyltransferase 1-associated protein 1 antibody; DNMAP1 antibody; DNMT1-associated protein 1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human DNA methyltransferase 1-associated protein 1 protein (250-300AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The DMAP1 Antibody (Product code: CSB-PA873611LA01HU) is Non-conjugated. For DMAP1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA873611LB01HU	DMAP1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA873611LC01HU	DMAP1 Antibody, FITC conjugated
Biotin	CSB-PA873611LD01HU	DMAP1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX-mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Participates in the nuclear localization of URI1 and increases its transcriptional corepressor activity.

Gene References into Functions

A novel tumour suppressor gene, MDGA2, which is frequently inactivated by promoter methylation in Gastric cancer, exerts a tumour suppressive function by cooperating with DMAP1 to activate the p53/p21 signalling cascade. PMID: 26206665
Targeted knockdown of human DNA methyltransferase 1 expression restores the expression levels of tumor suppressor genes, thus inhibiting the proliferation of cholangiocarcinoma cell line cells. PMID: 24361215
These results suggest that DMAP1 is a critical regulator of ATM activity and function PMID: 23318425
positive staining for DNMT1 in clinical cancer specimens was significantly linked to lower rates of response to treatments and shorter survival of patients with pharyngeal cancer. PMID: 21284050
Knocking down DMAP1 caused hypomethylation of the DNA repair products. PMID: 20864525
MMTR is an intrinsic negative cell cycle regulator that modulates the CAK kinase activity via interaction with MAT1. PMID: 20920467
RGS6 inhibited the transcriptional repressor activity of DMAP1. PMID: 14734556

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Subcellular Location

Nucleus. Cytoplasm. Note=Targeted to replication foci throughout S phase by DNMT1.

Database Links

HGNC: 18291

OMIM: 605077

KEGG: hsa:55929

STRING: 9606.ENSP00000312697

UniGene: Hs.8008

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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