DMGDH Antibody

Code CSB-PA883415LA01HU
Size US$299
Image
  • Western Blot
    Positive WB detected in: Mouse liver tissue, Mouse kidney tissue
    All lanes: DMGDH antibody at 6.7μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 97, 46 kDa
    Observed band size: 97 kDa

  • Immunofluorescence staining of HepG2 cells with CSB-PA883415LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC image of CSB-PA883415LA01HU diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA883415LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) DMGDH Polyclonal antibody
Uniprot No. Q9UI17
Target Names DMGDH
Alternative Names Dimethylglycine dehydrogenase antibody; Dimethylglycine dehydrogenase; mitochondrial antibody; Dmgdh antibody; M2GD_HUMAN antibody; ME2GLYDH antibody; mitochondrial antibody
Raised in Rabbit
Species Reactivity Human, Mouse
Immunogen Recombinant Human Dimethylglycine dehydrogenase, mitochondrial protein (429-524AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:200-1:500
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Catalyzes the demethylation of N,N-dimethylglycine to sarcosine. Also has activity with sarcosine in vitro.
Gene References into Functions
  1. In nonalcoholic fatty liver disease patient, the missense variant p.Ser646Pro (rs1805074) in DMGDH gene was significantly associated with disease severity and circulating levels of dimethylglycine. PMID: 27614103
  2. The structure-based analysis provided new insights into the kinetic properties of dimethylglycine dehydrogenase in particular with respect to oxygen reactivity. PMID: 27486859
  3. In agreement with previous studies, we show that the genetic variant rs921943 in DMGDH is significantly associated with selenium status in United Kingdom pregnant women. PMID: 26675765
  4. Genetic variation of DMGDH was associated with higher plasma insulin, increased insulin resistance and increased risk of incident diabetes. PMID: 25795213
  5. analysis of dimethylglycine dehydrogenase deficiency associated with pathogenic variant H109R PMID: 18937046

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Involvement in disease DMGDH deficiency (DMGDHD)
Subcellular Location Mitochondrion
Protein Families GcvT family
Database Links

HGNC: 24475

OMIM: 605849

KEGG: hsa:29958

STRING: 9606.ENSP00000255189

UniGene: Hs.655653

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