DOHH Antibody

Datasheet
Code CSB-PA880104LA01HU
See More Details
Size US$299
Uniprot No. Q9BU89
Image
  • Immunofluorescence staining of PC-3 cells with CSB-PA880104LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunogen Recombinant Human Deoxyhypusine hydroxylase protein (79-159AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IF; Recommended dilution: IF:1:50-1:200
Relevance Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Deoxyhypusine hydroxylase (hDOHH) (EC 1.14.99.29) (Deoxyhypusine dioxygenase) (Deoxyhypusine monooxygenase) (HEAT-like repeat-containing protein 1), DOHH, HLRC1
Immunogen Species Human
Protocols ELISA Protocol
Immunofluorescence (IF) Protocol
Research Area Signal Transduction
Target Names DOHH
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.
Protein Families Deoxyhypusine hydroxylase family
Database Links

HGNC: 28662

OMIM: 611262

KEGG: hsa:83475

STRING: 9606.ENSP00000250937

UniGene: Hs.515064

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