DPPA3 Antibody

Datasheet

Code	CSB-PA744290LA01HU
Size	US$166
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Image	IHC image of CSB-PA744290LA01HU diluted at 1:400 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) DPPA3 Polyclonal antibody

Uniprot No.

Q6W0C5

Target Names

DPPA3

Alternative Names

Compaction associated protein 1 antibody; Developmental pluripotency associated 3 antibody; Developmental pluripotency associated protein 3 antibody; Developmental pluripotency-associated protein 3 antibody; Dppa3 antibody; DPPA3_HUMAN antibody; PGC7 antibody; Stella related protein antibody; Stella-related protein antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Developmental pluripotency-associated protein 3 protein (1-159AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The DPPA3 Antibody (Product code: CSB-PA744290LA01HU) is Non-conjugated. For DPPA3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA744290LB01HU	DPPA3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA744290LC01HU	DPPA3 Antibody, FITC conjugated
Biotin	CSB-PA744290LD01HU	DPPA3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis.

Gene References into Functions

Super-enhancers at the Nanog locus differentially regulate neighboring pluripotency-associated genes, in particular, DPPA3. PMID: 27681417
We conclude that heterogeneous population of the HEK293T cells might be easily shifted towards expression of the pluripotency markers by ectopic expression of the SON factors or by growth in serum depleted media. PMID: 27794480
Data suggest enforced expression of Stella into normal cells induces not only global DNA demethylation but also neoplastic transformation and increases metastatic ability; these changes involve altered regulation of neoplastic gene expression. PMID: 26608031
Considering that impaired DNA methylation profiles are associated with various developmental or disease phenomena, Stella may be a powerful tool with which to study the biological effects of global DNA hypomethylation PMID: 25280994
a role for STELLA in facilitating germline and endodermal differentiation of human ES cells PMID: 23457636
human adult tissue-specific transcripts originate via retrotransposition of the pluripotent cell-specific gene, Dppa3 PMID: 16291741
These results demonstrate that PGC7/Stella is indispensable for the maintenance of methylation involved in epigenetic reprogramming after fertilization. PMID: 17143267
In mice, this gene may play a role in oogenesis and primordial germ cell development. PMID: 11900980
In mice, a targeted mutation results in reduced female fertility and may function as a mammalian maternal effect gene. PMID: 14654002
In mice, knockout experiments show this gene may be a maternal factor in cleavage stage embryogenesis. PMID: 15018652

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Subcellular Location

Nucleus. Cytoplasm.

Tissue Specificity

Low expression in testis, ovary and thymus. Expressed in embryonic stem and carcinoma cells. Highly expressed in testicular germ cell tumors.

Database Links

HGNC: 19199

OMIM: 608408

KEGG: hsa:359787

STRING: 9606.ENSP00000339250

UniGene: Hs.131358

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Cell markers Target Therapy

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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