EAPP Antibody

Code CSB-PA007353LA01HU
Size US$166
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Image
  • Western Blot
    Positive WB detected in: 293 whole cell lysate
    All lanes: EAPP antibody at 3.2µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 33 kDa
    Observed band size: 33 kDa

  • IHC image of CSB-PA007353LA01HU diluted at 1:400 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) EAPP Polyclonal antibody
Uniprot No.
Target Names
EAPP
Alternative Names
1810011O16Rik antibody; BM-036 antibody; BM036 antibody; C14orf11 antibody; E2F-associated phosphoprotein antibody; EAPP antibody; EAPP_HUMAN antibody; RGD1309624 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human E2F-associated phosphoprotein protein (1-285AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The EAPP Antibody (Product code: CSB-PA007353LA01HU) is Non-conjugated. For EAPP Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA007353LB01HU EAPP Antibody, HRP conjugated ELISA
FITC CSB-PA007353LC01HU EAPP Antibody, FITC conjugated
Biotin CSB-PA007353LD01HU EAPP Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:200-1:500
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
May play an important role in the fine-tuning of both major E2F1 activities, the regulation of the cell-cycle and the induction of apoptosis. Promotes S-phase entry, and inhibits p14(ARP) expression.
Gene References into Functions
  1. Independently of EAPP, E2F1 also increases the activity of the MDR1 promoter. PMID: 23542036
  2. results indicate that the level of EAPP is critical for cellular homeostasis PMID: 21258403
  3. EAPP modulates E2F-regulated transcription, stimulates proliferation, and may be involved in the malignant transformation of cells. PMID: 15716352
  4. Regulation of the EAPP promoter by GC-box binding proteins is reported. PMID: 18588995
Subcellular Location
Cytoplasm. Nucleus.
Tissue Specificity
Ubiquitously expressed. Highest levels in heart, placenta, skeletal muscle and pancreas. Lower levels in brain, lung and kidney. In the brain, expressed in all regions with high levels in the cerebellum and cerebral cortex. Expressed in COS1 and transform
Database Links

HGNC: 19312

OMIM: 609486

KEGG: hsa:55837

STRING: 9606.ENSP00000250454

UniGene: Hs.433269

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