ENPP7 Antibody

Datasheet

Code	CSB-PA751012LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: K562 whole cell lysate, HeLa whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Mouse heart tissue, Mouse kidney tissue All lanes: ENPP7 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa Immunofluorescence staining of HepG2 cells with CSB-PA751012LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ENPP7 Polyclonal antibody

Uniprot No.

Q6UWV6

Target Names

ENPP7

Alternative Names

ENPP7; UNQ3077/PRO9912; Ectonucleotide pyrophosphatase/phosphodiesterase family member 7; E-NPP 7; NPP-7; Alkaline sphingomyelin phosphodiesterase; Intestinal alkaline sphingomyelinase; Alk-SMase

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 7 protein (22-216AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ENPP7 Antibody (Product code: CSB-PA751012LA01HU) is Non-conjugated. For ENPP7 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA751012LB01HU	ENPP7 Antibody, HRP conjugated	ELISA
FITC	CSB-PA751012LC01HU	ENPP7 Antibody, FITC conjugated
Biotin	CSB-PA751012LD01HU	ENPP7 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IF	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

Choline-specific phosphodiesterase that hydrolyzes sphingomyelin releasing the ceramide and phosphocholine and therefore is involved in sphingomyelin digestion, ceramide formation, and fatty acid (FA) absorption in the gastrointestinal tract. Has also phospholipase C activity and can also cleave phosphocholine from palmitoyl lyso-phosphatidylcholine and platelet-activating factor (PAF) leading to its inactivation. Does not have nucleotide pyrophosphatase activity. May promote cholesterol absorption by affecting the levels of sphingomyelin derived from either diet or endogenous sources, in the intestinal lumen.

Gene References into Functions

human alkaline sphingomyelinase crystal structure provides insights into substrate recognition PMID: 28292932
NPP7 activity and the ratio of 1.4/1.2 kb products in bile are significantly decreased in malignancy, particularly in cholangiocarcinoma. PMID: 25100243
The F275A mutation of NPP7 showed impaired catalytic function whereas L107F mutation showed enhanced catalytic activity. PMID: 22177013
predict the three-dimensional structure of NPP7 by homology modeling using a recently crystallized NPP from bacteria. Using the model, we studied the substrate specificity of the enzyme by docking. PMID: 20839774
identified the amino acid and cDNA sequences of human intestinal alk-SMase, and found that it is a novel ecto-enzyme related to the ecto-nucleotide phosphodiesterase family with specific features essential for its SMase activity PMID: 12885774
intestinal alkaline sphingomyelinase may have a one-exon deletion in colon cancer cells PMID: 15016655
alk-SMase activity is severely affected by defective N-glycosylation at 5 sites and by structural alterations of the putative metal-binding sites and the predicted active core. PMID: 15458386
Results describe the cloning of rat alkaline sphingomyelinase from rat intestine, comparison to the human sequence, adjustment of the putative protein in GenBank, and confirmation of the specific expression of the gene in the small intestine. PMID: 15708357
Alkaline sphingomyelinase hydrolyses and inactivates PAF by a phospholipase C activity, a novel function by which it may counteract the development of intestinal inflammation and colon cancer. PMID: 16255717

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Families

Nucleotide pyrophosphatase/phosphodiesterase family

Tissue Specificity

Detected in the colon (at protein level). Expressed in the duodenum, jejunum and liver and at low levels in the ileum. Expression was very low in the esophagus, stomach and colon.

Database Links

HGNC: 23764

OMIM: 616997

KEGG: hsa:339221

STRING: 9606.ENSP00000332656

UniGene: Hs.114084

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