FAM96B Antibody

Datasheet

Code	CSB-PA897544ESR2HU
Size	US$166
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Image	Western blot All lanes: FAM96B antibody at 7.09µg/ml Lane 1: Mouse gonadal tissue Lane 2: MCF-7 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: K562 whole cell lysate Lane 5: A375 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 18 kDa Observed band size: 18 kDa Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA897544ESR2HU at dilution of 1:100 Immunoprecipitating FAM96B in Hela whole cell lysate Lane 1: Rabbit control IgG instead of (1µg) instead of CSB-PA897544ESR2HU in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: CSB-PA897544ESR2HU (4µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) FAM96B Polyclonal antibody

Uniprot No.

Q9Y3D0

Target Names

FAM96B

Alternative Names

CGI 128 antibody; FAM96B antibody; Family with sequence similarity 96; member B antibody; HSPC118 antibody; Hypothetical protein LOC51647 antibody; MIP18 antibody; MIP18_HUMAN antibody; Mitotic spindle-associated MMXD complex subunit MIP18 antibody; MSS19-interacting protein of 18 kDa antibody; Protein FAM96B antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Mitotic spindle-associated MMXD complex subunit MIP18 protein (1-163AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. As a CIA complex component and in collaboration with CIAO1 and MMS19, binds to and facilitates the assembly of most cytosolic-nuclear Fe/S proteins. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD. Together with MMS19, facilitates the transfer of Fe-S clusters to the motor protein KIF4A, which ensures proper localization of KIF4A to mitotic machinery components to promote the progression of mitosis.

Gene References into Functions

Data suggest that CIA2B and MMS19 physically interact with C-terminus of viperin/RSAD2; CIAO1 appears to function as primary viperin-interacting protein; CIA2A binds to N-terminus of viperin in CIAO1-, CIA2B-, and MMS19-independent fashion. (CIA2B = metallochaperone CIA2B/FAM96B; MMS19 = transcription factor MMS19; CIAO1 = cytosolic iron-sulfur assembly component 1; CIA2A = metallochaperone CIA2A/Fam96a) PMID: 28615450
CIA2B-CIA1-MMS19 and CIA2A-CIA1 assist different branches of Fe/S protein assembly and intimately link this process to cellular iron regulation via IRP1 Fe/S cluster maturation and IRP2 stabilization. PMID: 23891004
Co-localization experiments by fluorescent confocal microscopy revealed that FAM96B colocalized with prelamin A in HEK-293 cells. PMID: 24041693
The mammalian proteins MMS19, MIP18, and ANT2 are involved in cytoplasmic iron-sulfur cluster protein assembly. PMID: 23150669
these findings suggest that FAM96B acts as a regulator of E2-2 through the control of its protein expression. PMID: 21722264

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Subcellular Location

Nucleus. Cytoplasm, cytoskeleton, spindle. Midbody.

Protein Families

MIP18 family

Database Links

HGNC: 24261

OMIM: 614778

KEGG: hsa:51647

STRING: 9606.ENSP00000387471

UniGene: Hs.9825

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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