Product Details

Uniprot No.

Target Names

FBXO5

Alternative Names

Early mitotic inhibitor 1 antibody; Emi 1 antibody; EMI1 antibody; F box only protein 5 antibody; F box protein 5 antibody; F box protein Fbx 5 antibody; F box protein Fbx5 antibody; F-box only protein 5 antibody; FBX5 antibody; FBX5_HUMAN antibody; Fbxo31 antibody; fbxo5 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Human FBXO5

Immunogen Species

Homo sapiens (Human)

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.

Tested Applications

ELISA,WB,IHC

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

Regulator of APC activity during mitotic and meiotic cell cycle. During mitotic cell cycle plays a role as both substrate and inhibitor of APC-FZR1 complex. During G1 phase, plays a role as substrate of APC-FZR1 complex E3 ligase. Then switches as an inhibitor of APC-FZR1 complex during S and G2 leading to cell-cycle commitment. As APC inhibitor, prevents the degradation of APC substrates at multiple levels: by interacting with APC and blocking access of APC substrates to the D-box coreceptor, formed by FZR1 and ANAPC10; by suppressing ubiquitin ligation and chain elongation by APC by preventing the UBE2C and UBE2S activities. Plays a role in genome integrity preservation by coordinating DNA replication with mitosis through APC inhibition in interphase to stabilize CCNA2 and GMNN in order to promote mitosis and prevent rereplication and DNA damage-induced cellular senescence. During oocyte maturation, plays a role in meiosis through inactivation of APC-FZR1 complex. Inhibits APC through RPS6KA2 interaction that increases FBXO5 affiniy for CDC20 leading to the metaphase arrest of the second meiotic division before fertilization. Controls entry into the first meiotic division through inactivation of APC-FZR1 complex. Promotes migration and osteogenic differentiation of mesenchymal stem cells.

Gene References into Functions

demonstration using human cell models that cell-cycle commitment is mediated by an EMI1-APC/C(CDH1) dual-negative feedback switch, in which EMI1 is both a substrate and an inhibitor of APC/C(CDH1) PMID: 29875408
Both isoforms of FBXO5 promoted the migration and osteogenic differentiation potential of human periodontal ligament stem cells. PMID: 29850565
Results from a study on gene variability markers in early-stage human embryos shows that FBXO5 is a putative variability marker for the 3-day, 8-cell embryo stage. PMID: 26288249
The fact that Emi1 overexpression promotes chromosome instability (CIN) and the formation of solid cancers in vivo indicates that Emi1 overexpression actively drives solid tumorigenesis. These novel mechanistic insights have important clinical implications. PMID: 27065322
Examined eoffect of Emi1 over-expression on Skp2 expression in breast cancer. Found expression of Emi1 was positively related with Skp2 expression; Emi1 expression correlated significantly with histologic grade. Skp2 expression obtained similar results. PMID: 24277465
Human papillomavirus type 16 E7 expression causes increased EMI1 mRNA expression and also inhibits EMI1 degradation. PMID: 24074588
The C-terminal domain inhibits multiple APC/C(CDH1) functions. The intrinsically disordered D-box, linker & tail elements, & a structured Zn-binding domain synergistically block the substrate-binding site & inhibit ubiquitin-chain elongation. PMID: 23708605
Emi1 depletion enhances the sensitivity of cancer cells to doxorubicin and x-ray irradiation. PMID: 23645673
Emi1 expression (>5%) was seen in 23.3% of ovarian clear cell carcinoma, and associated with high FIGO grades and poor overall survival PMID: 23202783
Emi1 participates in human hepatocellular carcinoma (HCC) cell proliferation and that progression is controlled by anaphase-promoting complex/cyclosome (APC/C) inhibition, which stabilized Skp2 and enabled p27(kip1) degradation. PMID: 22995332
the ability of Emi1 to inhibit APC/C is negatively regulated by CDKs PMID: 21454540
Results suggest that Bcr-Abl increases Emi1 phosphorylation and stability to prevent Skp2 protein degradation via APC/Cdh1-induced ubiquitination and to enhance proliferation of CML cells. PMID: 20717963
These data suggest that E2F can activate both transcription of cyclin A and the hEmi1-dependent stabilization of APC(Cdh1) targets, such as cyclin A, to promote S phase entry. PMID: 11988738
Plk1 activates the anaphase promoting complex by directing the SCF-dependent destruction of Emi1 in prophase PMID: 15469984
loss of pRb repression of E2F-mediated transcription causing misregulation of Emi1 and APC/C substrates results in the generation of tetraploidy and proliferation of genomically unstable cells in the absence of normal p53 function PMID: 16861914
Emi1 associates in a complex with the anaphase-promoting complex/cyclosome (APC/C) and Cdh1; Emi1 binds to the APC/C via a conserved C-terminal destruction (D)-box and can compete for APC/C-substrate interaction. PMID: 16921029
bservations reveal a novel mechanism for the control of entry into the first meiotic division: an Emi1-dependent inhibition of APC(Cdh1). PMID: 17190794
These data suggest that Emi1 plays a critical role in preserving genome integrity by blocking rereplication, revealing a previously unrecognized function of this inhibitor of anaphase-promoting complex/cyclosome. PMID: 17234884
Emi1 plays a crucial role in the cell cycle to couple DNA replication with mitosis PMID: 17485488
critical spindle pole-associated mechanism, called the END (Emi1/NuMA/dynein-dynactin) network, spatially restricts APC/C activity in early mitosis PMID: 17609108
We investigated Emi1 protein expression in ovarian neoplasms PMID: 18204430
Emi1 down-regulation and APC activation leads to stable p21-dependent G2 arrest after DNA damage. PMID: 19211842
Results underscore the crucial role of cyclin A2-CDK2 in regulating the PLK1-SCF(beta-TrCP1)-EMI1-APC/C axis and CDC6 to trigger genome reduplication after the activity of CDK1 is suppressed. PMID: 19822658