FBXW5 Antibody

Datasheet

Code	CSB-PA846577LA01HU
Size	US$166
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Image	Western blot All lanes: FBXW5 antibody at 2μg/ml + PC-3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 64, 43 kDa Observed band size: 64 kDa Immunohistochemistry of paraffin-embedded human appendix tissue using CSB-PA846577LA01HU at dilution of 1:100 Immunofluorescent analysis of Hela cells using CSB-PA846577LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) FBXW5 Polyclonal antibody

Uniprot No.

Q969U6

Target Names

FBXW5

Alternative Names

FBXW5 antibody; FBW5 antibody; PP3971F-box/WD repeat-containing protein 5 antibody; F-box and WD-40 domain-containing protein 5 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human F-box/WD repeat-containing protein 5 protein (265-494AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The FBXW5 Antibody (Product code: CSB-PA846577LA01HU) is Non-conjugated. For FBXW5 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA846577LB01HU	FBXW5 Antibody, HRP conjugated	ELISA
FITC	CSB-PA846577LC01HU	FBXW5 Antibody, FITC conjugated
Biotin	CSB-PA846577LD01HU	FBXW5 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Substrate recognition component of both SCF (SKP1-CUL1-F-box protein) and DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes. Substrate recognition component of the SCF(FBXW5) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of SASS6 during S phase, leading to prevent centriole reduplication. The SCF(FBXW5) complex also mediates ubiquitination and degradation of actin-regulator EPS8 during G2 phase, leading to the transient degradation of EPS8 and subsequent cell shape changes required to allow mitotic progression. Substrate-specific adapter of the DCX(FBXW5) E3 ubiquitin-protein ligase complex which mediates the polyubiquitination and subsequent degradation of TSC2. May also act as a negative regulator of MAP3K7/TAK1 signaling in the interleukin-1B (IL1B) signaling pathway.

Gene References into Functions

oxidative stress induces Tnfaip8 l1/Oxi-beta, which results in increased autophagy by its exclusive binding with FBXW5 to stabilize TSC2 PMID: 24444419
DLC1 was ubiquitinated and degraded by cullin 4A-RING ubiquitin ligase (CRL4A) complex interaction with DDB1 and the FBXW5 substrate receptor. PMID: 24082123
Identify Fbxw5-driven fluctuation of Eps8 levels as an important mechanism that contributes to cell-shape changes during entry into-and exit from-mitosis. PMID: 23314863
Fbxw5 enhances sumoylation of nuclear c-Myb PMID: 22910413
FBXW5 levels are controlled by the anaphase-promoting (APC/C) complex, which targets FBXW5 for degradation during mitosis and G1, thereby helping to reset the centrosome duplication machinery. PMID: 21725316
Results indicate that FBW5-DDB1-CUL4-ROC1 is an E3 ubiquitin ligase regulating TSC2 protein stability and TSC complex turnover. PMID: 18381890
These results suggest that FBXW5 negatively regulates TAK1 in the IL-1beta signaling pathway. PMID: 19232515

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Subcellular Location

Cytoplasm.

Protein Families

FBXW5 family

Database Links

HGNC: 13613

OMIM: 609072

KEGG: hsa:54461

STRING: 9606.ENSP00000313034

UniGene: Hs.522507

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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