GATAD2A Antibody

Datasheet

Code	CSB-PA803156LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate, Rat stomach tissue All lanes: GATAD2A antibody at 2.9μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 69, 66 kDa Observed band size: 69 kDa IHC image of CSB-PA803156LA01HU diluted at 1:800 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA803156LA01HU diluted at 1:800 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) GATAD2A Polyclonal antibody

Uniprot No.

Q86YP4

Target Names

GATAD2A

Alternative Names

FLJ20085 antibody; FLJ21017 antibody; GATA zinc finger domain containing 2A antibody; GATA zinc finger domain containing protein 2A antibody; GATA zinc finger domain-containing protein 2A antibody; GATAD2A antibody; Hp66 alpha antibody; Hp66alpha antibody; OTTHUMP00000201911 antibody; OTTHUMP00000201912 antibody; P66 alpha antibody; P66A_HUMAN antibody; p66alpha antibody; Transcriptional repressor p66 alpha antibody; Transcriptional repressor p66-alpha antibody; Transcriptional repressor p66alpha antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human Transcriptional repressor p66-alpha protein (170-241AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The GATAD2A Antibody (Product code: CSB-PA803156LA01HU) is Non-conjugated. For GATAD2A Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA803156LB01HU	GATAD2A Antibody, HRP conjugated	ELISA
FITC	CSB-PA803156LC01HU	GATAD2A Antibody, FITC conjugated
Biotin	CSB-PA803156LD01HU	GATAD2A Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Transcriptional repressor. Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2B.

Gene References into Functions

The MYND domain of ZMYND8 directly interacts with PPPLPhi motifs in the NuRD subunit GATAD2A. PMID: 27732854
Results showed that suppression of GATAD2A attenuated thyroid cancer cell proliferation and colony formation and promoted apoptosis suggesting it as an essential factor in thyroid cancer cell pathogenesis. PMID: 28260108

Subcellular Location

Nucleus speckle.

Tissue Specificity

Ubiquitous, both in fetal and adult tissues.

Database Links

HGNC: 29989

OMIM: 614997

KEGG: hsa:54815

STRING: 9606.ENSP00000351552

UniGene: Hs.118964

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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