GCNA Antibody

Datasheet
Code CSB-PA836285LA01HU
See More Details
Size US$299
Uniprot No. Q96QF7
Image
  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate
    All lanes: GCNA antibody at 4.3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 77 kDa
    Observed band size: 77 kDa

  • IHC image of CSB-PA836285LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunogen Recombinant Human Acidic repeat-containing protein (364-443AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500
Relevance nucleus, DNA demethylation
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Acidic repeat-containing protein (Germ cell nuclear antigen), GCNA, ACRC
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Research Area Cell Biology
Target Names GCNA
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Subcellular Location Nucleus
Protein Families Serine-aspartate repeat-containing protein (SDr) family
Tissue Specificity Expressed in germ cells of the testis (at protein level) (PubMed:27718356). Detected in skeletal muscle, liver, kidney, pancreas, heart, lung and brain (PubMed:11714101).
Database Links

HGNC: 15805

OMIM: 300369

KEGG: hsa:93953

STRING: 9606.ENSP00000362799

UniGene: Hs.135167

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