GCNA Antibody

Code CSB-PA836285LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. Q96QF7
  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate
    All lanes: GCNA antibody at 4.3μg/ml
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 77 kDa
    Observed band size: 77 kDa

  • IHC image of CSB-PA836285LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunogen Recombinant Human Acidic repeat-containing protein (364-443AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500
Relevance nucleus, DNA demethylation
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Acidic repeat-containing protein (Germ cell nuclear antigen), GCNA, ACRC
Immunogen Species Human
Research Area Cell Biology
Target Names GCNA
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Subcellular Location Nucleus
Protein Families Serine-aspartate repeat-containing protein (SDr) family
Tissue Specificity Expressed in germ cells of the testis (at protein level) (PubMed:27718356). Detected in skeletal muscle, liver, kidney, pancreas, heart, lung and brain (PubMed:11714101).
Database Links

HGNC: 15805

OMIM: 300369

KEGG: hsa:93953

STRING: 9606.ENSP00000362799

UniGene: Hs.135167

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