GLIS3 Antibody

Datasheet

Code	CSB-PA839863LA01HU
Size	US$166
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Image	IHC image of CSB-PA839863LA01HU diluted at 1:300 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CSB-PA839863LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) GLIS3 Polyclonal antibody

Uniprot No.

Q8NEA6

Target Names

GLIS3

Alternative Names

GLIS3 antibody; ZNF515 antibody; Zinc finger protein GLIS3 antibody; GLI-similar 3 antibody; Zinc finger protein 515 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Zinc finger protein GLIS3 protein (527-663AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The GLIS3 Antibody (Product code: CSB-PA839863LA01HU) is Non-conjugated. For GLIS3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA839863LB01HU	GLIS3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA839863LC01HU	GLIS3 Antibody, FITC conjugated
Biotin	CSB-PA839863LD01HU	GLIS3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Acts as both a repressor and activator of transcription. Binds to the consensus sequence 5'-GACCACCCAC-3'.

Gene References into Functions

Data show that loss of zinc finger protein GLIS3 (GLIS3) causes beta-cell death. PMID: 29992946
Data did not show any associations of GLI similar 3 protein (GLIS3) gene polymorphisms rs806052, rs143051164, and rs149840771 with carbohydrate metabolism disorders among patients with maturity onset diabetes of the young (MODY) and type 2 diabetes mellitus (DM2) in Russia. PMID: 29606121
Individually the rs7020673 and rs10758593 SNPs are not significantly associated with T1DM but seem to interact in the predisposition for this disease. PMID: 28597135
Given the role of GLIS3 in transcriptional activation and repression during embryogenesis, in humans, GLIS3 mutations present with multisystem involvement that also includes renal cystic dysplasia, progressive liver fibrosis and osteopenia. Thyroid findings in GLIS3 patients include thyroid aplasia, diminished colloid with interstitial fibrosis at post-mortem.[review] PMID: 28648506
GLIS3 polymorphism is not associated with Dermatomyositis /Polymyositis in the Chinese Han population PMID: 28846454
we describe the common facial dysmorphism consisting of bilateral low-set ears, depressed nasal bridge with overhanging columella, elongated, upslanted palpebral fissures, persistent long philtrum with a thin vermilion border of the upper lip in a cohort of seven patients with GLIS3 mutations and report the emergence of a distinct, probably recognizable facial gestalt in this group which evolves with age. PMID: 27148679
It may play a role in a number of physiological processes controlled by Glis3. PMID: 26147758
New findings with GLIS3 phenotype including craniosynostosis, hiatus hernia, atrial septal defect, splenic cyst, and choanal atresia and confirm further cases with sensorineural deafness and exocrine pancreatic insufficiency. PMID: 26259131
analysis of a GLIS3 variant that may have a role in resistance to Japanese type 1 diabetes PMID: 23856252
The present data suggest that altered expression of the candidate gene GLIS3 may contribute to both type 1 and 2 type diabetes by favouring beta cell apoptosis PMID: 23737756
Alleles of single nucleotide polymorphisms in GLIS3 and ADCY5 may confer risk of type 2 diabetes. PMID: 21949744
the associations of GLIS3-rs7034200 and CRY2-rs11605924 with fasting glucose, beta cell function, and type 2 diabetes PMID: 21747906
Glis3 interacts with Suppressor of Fused (SUFU) PMID: 21543335
results demonstrate a major role for GLIS3 in the development of pancreatic beta cells and the thyroid, eye, liver and kidney PMID: 16715098

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Involvement in disease

Diabetes mellitus, neonatal, with congenital hypothyroidism (NDH)

Subcellular Location

Nucleus.

Protein Families

GLI C2H2-type zinc-finger protein family

Tissue Specificity

In the adult, expressed at high levels in the kidney and at lower levels in the brain, skeletal muscle, pancreas, liver, lung, thymus and ovary.

Database Links

HGNC: 28510

OMIM: 610192

KEGG: hsa:169792

STRING: 9606.ENSP00000371398

UniGene: Hs.162125

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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