GPLD1 Antibody

Datasheet
Code CSB-PA009721LA01HU
Size US$299
Uniprot No. P80108
Image
  • Western Blot
    Positive WB detected in: SH-SY5Y whole cell lysate
    All lanes: GPLD1 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 93, 20 kDa
    Observed band size: 93 kDa

  • IHC image of CSB-PA009721LA01HU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunogen Recombinant Human Phosphatidylinositol-glycan-specific phospholipase D protein (310-565AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200
Relevance This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Phosphatidylinositol-glycan-specific phospholipase D (PI-G PLD) (EC 3.1.4.50) (Glycoprotein phospholipase D) (Glycosyl-phosphatidylinositol-specific phospholipase D) (GPI-PLD) (GPI-specific phospholipase D), GPLD1, PIGPLD1
Immunogen Species Human
Research Area Cardiovascular
Target Names GPLD1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane.
Subcellular Location Secreted
Protein Families GPLD1 family
Database Links

HGNC: 4459

OMIM: 602515

KEGG: hsa:2822

STRING: 9606.ENSP00000230036

UniGene: Hs.533291

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