Human Phosphatidylinositol-glycan-specific phospholipase D(GPLD1) ELISA kit

Code CSB-EL009721HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P80108
Lead Time 7-14 working days
Abbreviation GPLD1
Target Name glycosylphosphatidylinositol specific phospholipase D1
Alias GPIPLD, GPIPLDM, MGC22590, PIGPLD, PIGPLD1, GPI-specific phospholipase D|glycoprotein phospholipase D|glycosylphosphatIdylinositol-specific phospholipase D|glycosylphosphatidylinositol specific phos
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. This protein is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane.
HGNC 4459
RGD 631371
MGI 106604
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human GPLD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:200Average %90
Range %84-96
1:400Average %97
Range %91-103
1:800Average %103
Range %97-109
1:1600Average %89
Range %83-95
The recovery of human GPLD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10094-106
EDTA plasma (n=4)9591-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
2002.841 2.752 2.797 2.673
1002.564 2.402 2.483 2.359
502.135 1.965 2.050 1.926
251.358 1.355 1.357 1.233
12.50.691 0.744 0.718 0.594
6.250.371 0.395 0.383 0.259
3.120.225 0.226 0.226 0.102
00.125 0.123 0.124
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Function This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane.
Subcellular Location Secreted
Protein Families GPLD1 family
Database Links

HGNC: 4459

OMIM: 602515

KEGG: hsa:2822

STRING: 9606.ENSP00000230036

UniGene: Hs.533291

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