GRB14 Antibody

Datasheet

Code	CSB-PA900627
Size	US$166
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Image	The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA900627(GRB14 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA900627(GRB14 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200) Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Human fetal intestines tissue, Primary antibody: CSB-PA900627(GRB14 Antibody) at dilution 1/350 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

Q14449

Target Names

GRB14

Alternative Names

GRB14Growth factor receptor-bound protein 14 antibody; GRB14 adapter protein antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein of Human GRB14

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form

Liquid

Tested Applications

ELISA,WB,IHC

Recommended Dilution

Application	Recommended Dilution
ELISA	1:1000-1:2000
WB	1:200-1:1000
IHC	1:25-1:100

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal.

Gene References into Functions

We investigated the modification of air pollution and diabetes association by a genetic risk score covering 63 T2D genes. Five single variants near GRB14, UBE2E2, PTPRD, VPS26A and KCNQ1 showed nominally significant interactions with PM10 (P<0.05). Our results suggest that genetic risk for T2D may modify susceptibility to air pollution through alterations in insulin sensitivity. PMID: 27281273
The N-terminus of the BPS domain plays an important role in the regulation of human Grb14 and insulin receptor complex formation through phosphorylation, in addition to other domains. PMID: 28130417
Colorectal cancer patients with high GRB14 levels had a shorter survival and GRB14 was upregulated at an advanced clinical stage with enhanced tumor invasion and lymph node metastasis. PMID: 26965150
Phosphorylation of Grb14 BPS domain by GSK-3 correlates with complex forming of Grb14 and insulin receptor. PMID: 24535599
Genes within recently identified loci associated with waist-hip ratio (WHR) exhibit fat depot-specific mRNA expression, which correlates with obesity-related traits. Adipose tissue (AT) mRNA expression of 6 genes (TBX15/WARS2, STAB1, PIGC, ZNRF3, GRB14 PMID: 23670221
Modulation of mouse rod photoreceptor responses by Grb14 protein. PMID: 24273167
ANKRD55 rs459193 and GRB14 rs13389219 associate with insulin resistance. PMID: 23457408
Grb14 is the first negative regulator of CEACAM3-initiated bacterial phagocytosis and might help to focus granulocyte responses to the subcellular sites of pathogen-host cell contact PMID: 22948154
A new role for Grb14 in finely tuning receptor signaling and modulating thyroid cancer progression. PMID: 22158039
serves as an adaptor protein to recruit 3-phosphoinositede-dependent kinase-1 to activated insulin receptor, thus promoting Akt phosphorylation and transduction of the insulin signal PMID: 15210700
regulation of Grb14 expression levels in response to hormonal stimuli, and are consistent with its role as a repressor of insulin signaling where it is induced as a negative feedback mechanism. PMID: 15372466
Grb14 may regulate signalling through the insulin receptor by controlling its tyrosine dephosphorylation in a site-specific manner. PMID: 16582879
GRB14 is a weight-loss-responsive gene in skeletal muscle. Its observed transcriptional modulation may improve insulin signaling, with weight loss. PMID: 16849634
Grb14 regulates insulin action at two levels, through insulin receptors binding and by interfering with downstream pathways. PMID: 19359342

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Subcellular Location

Cytoplasm. Endosome membrane; Peripheral membrane protein.

Protein Families

GRB7/10/14 family

Tissue Specificity

Expressed at high levels in the liver, kidney, pancreas, testis, ovary, heart and skeletal muscle.

Database Links

HGNC: 4565

OMIM: 601524

KEGG: hsa:2888

STRING: 9606.ENSP00000263915

UniGene: Hs.411881

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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