HIST1H1C (Ab-164) Antibody

Datasheet
Code CSB-PA010378OA164nphHU
Size US$319
Uniprot No. P16403
Image
  • Western Blot
    Positive WB detected in: Jurkat whole cell lysate
    All lanes: HIST1H1C antibody at 1.7µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 22 kDa
    Observed band size: 22 kDa

  • IHC image of CSB-PA010378OA164nphHU diluted at 1:50 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H1C (CSB-PA010378OA164nphHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

Immunogen Peptide sequence around site of Thr (164) derived from Human Histone H1.2
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, ChIP; Recommended dilution: WB:1:50-1:500, IHC:1:20-1:200
Relevance Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias Histone H1.2 (Histone H1c) (Histone H1d) (Histone H1s-1), HIST1H1C, H1F2
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Research Area Epigenetics and Nuclear Signaling
Target Names HIST1H1C
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Subcellular Location Nucleus, Chromosome
Protein Families Histone H1/H5 family
Database Links

HGNC: 4716

OMIM: 142710

KEGG: hsa:3006

STRING: 9606.ENSP00000339566

UniGene: Hs.7644

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