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HIST1H2AG (Ab-118) Antibody

Datasheet
Code CSB-PA010389OA118nme1HU
Size US$175
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Image
  • Western Blotting(WB) - HIST1H2AG (Ab-118) Antibody

    Western Blot
    Positive WB detected in: NIH/3T3 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate
    All lanes: HIST1H2AG antibody at 1µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 15 kDa
    Observed band size: 15 kDa

  • Immunoprecipitation(IP) - HIST1H2AG (Ab-118) Antibody

    Immunoprecipitating HIST1H2AG in NIH/3T3 whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-PA010389OA118nme1HU in NIH/3T3 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-PA010389OA118nme1HU (5µg) + NIH/3T3 whole cell lysate (500µg)
    Lane 3: NIH/3T3 whole cell lysate (20µg)

  • Chromatin Immunoprecipitation(ChIP) - HIST1H2AG (Ab-118) Antibody

    Chromatin Immunoprecipitation Hela (106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2AG (CSB-PA010389OA118nme1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) HIST1H2AG Polyclonal antibody
Uniprot No.
P0C0S8
Target Names
HIST1H2AG
Alternative Names
H2AC11 antibody; H2AFP antibody; HIST1H2AG; antibody; H2AC13 antibody; H2AFC antibody; HIST1H2AI; antibody; H2AC15 antibody; H2AFD antibody; HIST1H2AK; antibody; H2AC16 antibody; H2AFI antibody; HIST1H2AL; antibody; H2AC17 antibody; H2AFN antibody; HIST1H2AMHistone H2A type 1 antibody; H2A.1 antibody; Histone H2A/ptl antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Peptide sequence around site of Lys (118) derived from Human Histone H2A type 1
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IP, ChIP
Recommended Dilution
Application Recommended Dilution
WB 1:100-1:1000
IP 1:200-1:2000
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Immunoprecipitation (IP) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Subcellular Location
Nucleus. Chromosome.
Protein Families
Histone H2A family
Database Links

HGNC: 4737

OMIM: 602787

KEGG: hsa:8329

STRING: 9606.ENSP00000352627

UniGene: Hs.134999

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