HOOK3 Antibody

Code CSB-PA769790LA01HU
Size US$166
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Image
  • Western Blot
    Positive WB detected in: 293 whole cell lysate
    All lanes: HOOK3 antibody at 4.9µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 84 kDa
    Observed band size: 84 kDa

  • IHC image of CSB-PA769790LA01HU diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunoprecipitating HOOK3 in 293 whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-PA769790LA01HU in 293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-PA769790LA01HU (6µg) + 293 whole cell lysate (500µg)
    Lane 3: 293 whole cell lysate (20µg)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) HOOK3 Polyclonal antibody
Uniprot No.
Target Names
HOOK3
Alternative Names
FLJ31058 antibody; h-hook3 antibody; hHK3 antibody; HK3 antibody; hook3 antibody; HOOK3_HUMAN antibody; OTTHUMP00000229200 antibody; Protein Hook homolog 3 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Protein Hook homolog 3 protein (357-455AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The HOOK3 Antibody (Product code: CSB-PA769790LA01HU) is Non-conjugated. For HOOK3 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA769790LB01HU HOOK3 Antibody, HRP conjugated ELISA
FITC CSB-PA769790LC01HU HOOK3 Antibody, FITC conjugated
Biotin CSB-PA769790LD01HU HOOK3 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:200-1:500
IP 1:200-1:2000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Probably serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking. Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). FHF complex promotes the distribution of AP-4 complex to the perinuclear area of the cell.; (Microbial infection) May serve as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking.
Gene References into Functions
  1. This work reveals the structural details of Hook3's interaction with dynein and offers insight into how cargo adaptors form processive dynein-dynactin motor complexes. PMID: 27482052
  2. Single-molecule motility assays using total internal reflection fluorescence microscopy indicate that both Hook1 and Hook3 effectively activate cytoplasmic dynein, inducing longer run lengths and higher velocities than the previously characterized dynein activator bicaudal D2 (BICD2). PMID: 27365401
  3. results of our study identify HOOK3 as a strong candidate prognostic marker with a possible role in maintaining genomic integrity in prostate cancer, which may have potential for inclusion into clinical routine assays PMID: 26230842
  4. Results found that downregulation of Hook3 expression slows down endosomal transport and increases beta-amyloid production suggesting that Hook3 might play a critical role in pathogenic events exacerbating Alzheimer disease. PMID: 25799409
  5. Hook3 is recruited to pericentriolar satellites through interaction with pericentriolar material 1 protein PCM1. PMID: 20152126
  6. Salmonella SpiC targets the function of Hook3, a mammalian protein implicated in cellular trafficking. PMID: 12950921
  7. Hook3 interacts with a cytoplasmic domain of scavenger receptor A PMID: 17237231
  8. A fusion product between exon 11 of HOOK3 and exon 12 of RET gene was identified by 5'RACE, and the presence of chimeric HOOK3-RET protein of 88 kDa was detected by western blot analysis with an anti-RET antibody. PMID: 17639057

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Subcellular Location
Cytoplasm, cytoskeleton. Golgi apparatus.
Protein Families
Hook family
Database Links

HGNC: 23576

OMIM: 607825

KEGG: hsa:84376

STRING: 9606.ENSP00000305699

UniGene: Hs.162852

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