HOOK3 Antibody

Datasheet
Code CSB-PA769790LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. Q86VS8
Image
  • Western Blot
    Positive WB detected in: 293 whole cell lysate
    All lanes: HOOK3 antibody at 4.9µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 84 kDa
    Observed band size: 84 kDa

  • IHC image of CSB-PA769790LA01HU diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunoprecipitating HOOK3 in 293 whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-PA769790LA01HU in 293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-PA769790LA01HU (6µg) + 293 whole cell lysate (500µg)
    Lane 3: 293 whole cell lysate (20µg)

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
Immunogen Recombinant Human Protein Hook homolog 3 protein (357-455AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IP:1:200-1:2000
Relevance Probably serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (PubMed:25035494).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Protein Hook homolog 3 (h-hook3) (hHK3), HOOK3
Immunogen Species Human
Research Area Signal Transduction
Target Names HOOK3
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Probably serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track)
Subcellular Location Cytoplasm, cytoskeleton, Golgi apparatus
Protein Families Hook family
Database Links

HGNC: 23576

OMIM: 607825

KEGG: hsa:84376

STRING: 9606.ENSP00000305699

UniGene: Hs.162852

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