HSD3B7 Antibody

Code CSB-PA861999LA01HU
Size US$166
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  • Western Blot
    Positive WB detected in: Mouse liver tissue, Mouse kidney tissue
    All lanes: HSD3B7 antibody at 3.5μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 42, 22 kDa
    Observed band size: 42 kDa

  • Immunofluorescent analysis of HepG2 cells using CSB-PA861999LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) HSD3B7 Polyclonal antibody
Uniprot No.
Target Names
HSD3B7
Alternative Names
HSD3B7; 3 beta-hydroxysteroid dehydrogenase type 7; 3 beta-hydroxysteroid dehydrogenase type VII; 3-beta-HSD VII; 3-beta-hydroxy-Delta(5-C27 steroid oxidoreductase; C(27 3-beta-HSD; Cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human 3 beta-hydroxysteroid dehydrogenase type 7 protein (131-197AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The HSD3B7 Antibody (Product code: CSB-PA861999LA01HU) is Non-conjugated. For HSD3B7 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA861999LB01HU HSD3B7 Antibody, HRP conjugated ELISA
FITC CSB-PA861999LC01HU HSD3B7 Antibody, FITC conjugated
Biotin CSB-PA861999LD01HU HSD3B7 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. HSD VII is active against four 7-alpha-hydroxylated sterols. Does not metabolize several different C(19/21) steroids as substrates. Involved in bile acid synthesis. Plays a key role in cell positioning and movement in lymphoid tissues by mediating degradation of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC): 7-alpha,25-OHC acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells.
Gene References into Functions
  1. Novel Mutations in the 3beta-hydroxy-5-C27-steroid Dehydrogenase Gene (HSD3B7) in a Patient with Neonatal Cholestasis. PMID: 26712441
  2. Expression of steroid sulfated transporters and 3beta-HSD activity in endometrium of polycystic ovary syndrome PMID: 26450365
  3. Homozygosity mapping identifies a bile acid biosynthetic defect (3beta-HSD deficiency due to a frameshift mutation in HSD3B7) in an adult with cirrhosis of unknown etiology. PMID: 22095780
  4. Mutations in the HSD3B7 gene account for autosomal recessive neonatal cholestasis caused by 3[beta]-hydroxy-[DELTA]5-C27-steroid dehydrogenase/isomerase deficiency. PMID: 20531254
Involvement in disease
Congenital bile acid synthesis defect 1 (CBAS1)
Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
3-beta-HSD family
Database Links

HGNC: 18324

OMIM: 607764

KEGG: hsa:80270

STRING: 9606.ENSP00000297679

UniGene: Hs.460618

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